ANTIGENIC-COMPETITION IN A MULTIVALENT FOOT ROT VACCINE
ANTIGENIC-COMPETITION IN A MULTIVALENT FOOT ROT VACCINE
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DOI：
10.1016/0264-410x(94)90125-2
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发表时间：
1994-04-01
期刊：
影响因子：
5.5
通讯作者：
STEWART, DJ
中科院分区：
文献类型：
--
作者：
HUNT, JD;JACKSON, DC;STEWART, DJ
已经使用重组菌毛抗原研究了当不同血清群的菌毛抗原组合在用于腐烂足的多价疫苗中时发生的抗原竞争。我们的原型疫苗含有来自铜绿假单胞菌中表达的结节二齿菌九个血清群的药丸。接种这种多价疫苗的绵羊对腐蹄病的预防效果不如接种单价疫苗的绵羊。发现在接种了六种或更多密切相关的药丸的绵羊中，针对任何特定药丸血清群的凝集和总抗体特异性水平显着降低。这种效应对于凝集抗体更为明显，人们认为凝集抗体可以介导保护作用，但通过 ELISA 测量的总抗体水平也观察到了这种效应。抗原竞争与总抗原负载无关，因为十倍高剂量的单价药丸诱导高滴度抗体。此外，将疫苗分配到四个部位，每个部位引流到不同的淋巴结，也未能克服竞争。使用单特异性血清混合物进行的实验表明，这种现象不太可能是由于异源菌毛引起的过量交叉反应性非保护性抗体对血清群特异性保护性抗体的阻断所致。
The antigenic competition that occurs when pilus antigens of different serogroups are combined in multivalent vaccines for foot rot has been investigated using recombinant pilus antigens. Our prototype vaccine contains pill from nine serogroups of Dichelobacter nodosus which are expressed in Pseudomonas aeruginosa. Sheep inoculated with this multivalent vaccine were not as well protected against foot rot as those given the monovalent vaccine. Levels of agglutinating and total antibody specific far any particular pill serogroup were found to be significantly reduced in sheep vaccinated with six or more closely related pill. This effect was more pronounced for agglutinating antibody, which is thought to mediate protection, but was also observed with total antibody levels measured by ELISA. The antigenic competition was not associated with the total antigen load as a tenfold higher dose of monovalent pill induced high titres of antibody. Furthermore, distributing the vaccine to four sites, each draining to a different lymph node, failed to overcome the competition. Experiments with mixtures of monospecific sera indicate that the phenomenon is unlikely to be due to blocking of serogroup-specific protective antibodies by an excess of cross-reactive non-protective antibody elicited by heterologous pili.