IL-10 producing CD8+ T cells in human infection with Leishmania guyanensis
IL-10 producing CD8+ T cells in human infection with Leishmania guyanensis
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DOI：
10.1016/j.micinf.2007.04.009
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发表时间：
2007-07-01
影响因子：
5.8
通讯作者：
Launois, Pascal
中科院分区：
文献类型：
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作者：
Bourreau, Eliane;Ronet, Catherine;Launois, Pascal
细胞因子日益被认为是细胞内病原体免疫反应的重要组成部分。在本研究中，我们分析了未暴露的幼稚受试者和LCL患者在活体古雅利什曼原虫刺激后PBMC产生的转化生长因子-β、IL-10和干扰素-γ的情况。我们发现正常人和LCL患者都产生了干扰素-γ，IL-10仅在LCL患者中产生，而转化生长因子-β仅在幼稚受试者中产生。此外，在幼稚的受试者中，中和转化生长因子-β诱导了IL-10的产生。当转化生长因子-β被中和时，幼稚受试者产生的IL-10或LCL患者产生的IL-10不会改变干扰素-γ的产生，但会抑制活性氮的产生。对初治受试者中和转化生长因子-β后IL-10产生细胞的表型分析表明，它们是记忆性CD45RA(-)CD8(+)T细胞。在LCL患者中，产生IL-10的细胞既有CD45RA(-)CD4细胞，也有CD8(+)T细胞。应仔细评估这些产生IL-10的CD8(+)T细胞在疾病发展中的作用。(C)2007年爱思唯尔·马森公司。版权所有。
Cytokines are increasingly recognized as important components of the cellular immune responses to intracellular pathogens. In this study, we analyzed the production of TGF-beta, IL-10 and IFN-gamma by PBMC of unexposed naive subjects and LCL patients after stimulation with live Leishmania guyanensis (L.g.). We demonstrated that IFN-gamma is produced in controls and LCL patients, IL-10 only in LCL patients and TGF-beta only in naive subjects. Furthermore, in naive subjects, neutralization of TGF-beta induced IL-10 production. IL-10 produced in naive subjects when TGF-beta is neutralized or in LCL patients did not modify the IFN-gamma production but inhibit reactive nitrogen species production. Analysis of the phenotype of IL-10 producing cells in naive subjects when TGF-beta is neutralized clearly showed that they are memory CD45RA(-) CD8(+) T cells. In LCL patients, IL-10 producing cells are both CD45RA(-) CD4 and CD8(+) T cells. The role of these IL-10 producing CD8(+) T cells in the development of the diseases should be carefully evaluated. (c) 2007 Elsevier Masson SAS. All rights reserved.