Cloning and expression profiling of four antibacterial peptide genes from the bumblebee Bombus ignitus
Cloning and expression profiling of four antibacterial peptide genes from the bumblebee Bombus ignitus
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DOI：
10.1016/j.cbpb.2008.02.007
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发表时间：
2008-06-01
影响因子：
2.2
通讯作者：
Jin, Byung Rae
中科院分区：
文献类型：
--
作者：
Choi, Yong Soo;Choo, Young Moo;Jin, Byung Rae
从熊蜂（Bombusigarbae）中克隆了4个抗菌肽基因（apidaecin、hybridtaecin、abaecin和defensin），并对其cDNA序列和基因组结构进行了分析。比较分析表明，B. IgG1具有与迄今鉴定的其它蜜蜂抗菌肽相似的特性。四种抗菌肽基因在B脂肪体中的转录表达谱。免疫学工作者揭示了所有四种抗菌肽基因都以相似的方式被PBS注射或LPS刺激急性诱导，表明在感染或损伤时，来自不同种类的抗菌肽在单个昆虫中同时表达。(c)2008年爱思唯尔公司All rights reserved.
Four antibacterial peptide genes (apidaecin, hymenoptaecin, abaecin, and defensin) were cloned from the bumblebee Bombus ignitus, and cDNAs and their genomic structures were sequenced and characterized. Comparative analysis revealed that the four antibacterial peptides of B. ignitus had similar characteristics to other bee antibacterial peptides identified to date. The transcriptional expression profiles of the four antibacterial peptide genes in the fat body of B. ignitus workers revealed that all four antibacterial peptide genes were acutely induced in a similar manner by PBS injection or LPS stimulation, indicating that antibacterial peptides from various classes are simultaneously expressed in a single insect upon infection or injury. (c) 2008 Elsevier Inc. All rights reserved.