Role of occlusion in mouse masseter muscle acetylcholine receptor clustering
Role of occlusion in mouse masseter muscle acetylcholine receptor clustering
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闭塞在小鼠咬肌乙酰胆碱受体聚集中的作用
DOI：
10.1177/0022034513476038
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
MORI;M.;NARIYAMA;M.;ABO;T.;HIRAI;S.;OGAWA;T.;HAMADA;Y.;YAMANE;A. and ASADA;Y.
烟碱乙酰胆碱受体 (nAChR) 聚集是神经肌肉接头 (NMJ) 突触发生中的关键事件，用于将神经信号从运动神经元有效传输到骨骼肌。具有mitf基因突变的小眼小鼠（mi/mi）无法进行咬合，因为它的牙齿不会萌出。本研究试图以mi/mias为模型系统，阐明咬肌NMJ中nAChR聚集的闭塞作用。在 1 周龄的小鼠中，在母乳喂养 +/+ 和 mi/mi 之间，咬肌或腓肠肌中 nAChR 簇的碎片或体积没有观察到显着变化。在4周龄和12周龄的小鼠中，在+/+出现闭塞后，与+/+饲喂颗粒或粉状饮食的小鼠相比，在喂食粉状饮食的mi/mif的咬肌中观察到nAChR簇的过度破碎和体积下降，而在腓肠肌中，两种品系之间没有观察到这种差异。这些结果表明mi/mi咬肌NMJ中nAChR簇的形成异常，表明闭塞对于咬肌NMJ中nAChR簇的正常进展至关重要。
Nicotinic acetylcholine receptor (nAChR) clustering is a key event in the synaptogenesis of the neuromuscular junction (NMJ) for the efficient transmission of neural signals from motor neurons to skeletal muscle. The microphthalmic mouse (mi/mi) with a mutation in themitfgene cannot perform occlusion, because its teeth do not erupt. The present study attempted to elucidate the contribution of occlusion to the clustering of nAChR in the NMJ of the masseter, withmi/mias a model system. In mice at 1 week of age, no significant change in the fragmentation or volume of the nAChR cluster was observed in either the masseter or gastrocnemius between breast-fed +/+ andmi/mi. In mice at 4 and 12 weeks of age, after the occlusion emerged in the +/+, excessive fragmentation and volume decline in the nAChR cluster were observed in the masseter ofmi/mifed a powdered diet compared with +/+ fed a pellet or powdered diet, whereas, in the gastrocnemius, no such differences were observed between the 2 strains. These results indicate abnormal formation of the nAChR cluster in the NMJ of the masseter ofmi/mi, suggesting that occlusion is essential for the normal progress of nAChR clustering in the NMJ of the masseter.