Entomological Study on Transmission of Avian Malaria Parasites in a Zoological Garden in Japan: Bloodmeal Identification and Detection of Avian Malaria Parasite DNA From Blood-Fed Mosquitoes
Entomological Study on Transmission of Avian Malaria Parasites in a Zoological Garden in Japan: Bloodmeal Identification and Detection of Avian Malaria Parasite DNA From Blood-Fed Mosquitoes
复制标题
DOI：
10.1603/me10197
复制
发表时间：
2011-05-01
影响因子：
2.1
通讯作者：
Yukawa, Masayoshi
中科院分区：
文献类型：
--
作者：
Ejiri, Hiroko;Sato, Yukita;Yukawa, Masayoshi
在日本已发现几种圈养和野生鸟类感染各种鸟类血液原虫。我们调查了日本东京一个动物园的禽疟原虫的流行和传播，并利用聚合酶链反应确定了蚊子的血粉宿主。共收集了7种310只未喂食和140只吸血蚊子，使用扫网和CDC诱捕器。血粉鉴定表明，蚊子以17种鸟类和5种哺乳动物为食，其中包括圈养动物。对采集的禽血粉进行了蚊虫疟原虫检测，结果表明，淡色库蚊是本研究区禽疟原虫的主要传播媒介，部分圈养和野生鸟类可感染疟原虫。此外，还估计了吸血蚊采集点与其血源圈养动物所在地之间的距离。在笼内动物40 m范围内发现大多数含新鲜血粉的雌性动物，而在笼内宿主动物10 m至350 m范围内发现半妊娠和妊娠雌性动物。我们证明，吸血蚊子可以提供有用的信息，蚊子媒介物种的禽疟疾寄生虫，并允许非侵入性检测鸟类种群中的禽疟疾寄生虫的存在。
Several species of captive and wild birds have been found to be infected with various avian blood protozoa in Japan. We investigated the prevalence and transmission of avian malaria parasite and determined the bloodmeal hosts of mosquitoes collected in a zoological garden in Tokyo, Japan, by using the polymerase chain reaction. In total, 310 unfed and 140 blood-fed mosquitoes of seven species were collected by using sweep nets and CDC traps. Bloodmeal identification indicated that mosquitoes had fed on 17 avian and five mammalian species, including captive animals. The results of avian malaria parasite detection from mosquitoes with avian bloodmeals indicated that Culex pipiens pallens Coquillet is a main vector of avian Plasmodium in the current study site and that some captive and wild birds could be infected with avian malaria parasites. Furthermore, the distances between the collection site of blood-fed mosquitoes and the locations of their blood-source captive animals were estimated. Most females with fresh bloodmeals were found within 40 m of caged animals, whereas half-gravid and gravid females were found between 10 and 350 m from caged host animals. We demonstrated that blood-fed mosquitoes can provide useful information regarding the mosquito vector species of avian malaria parasites and allows for noninvasive detection of the presence of avian malaria parasites in bird populations.