Physical modeling of flow boiling in microchannels and its induced vitrification of biomaterials
Physical modeling of flow boiling in microchannels and its induced vitrification of biomaterials
复制标题
微通道中流动沸腾的物理模型及其诱导的生物材料玻璃化
DOI：
10.1016/j.ijheatmasstransfer.2014.12.063
复制
发表时间：
2015-04-01
影响因子：
5.2
通讯作者：
Gao, Dayong
中科院分区：
文献类型：
--
作者：
Zhou, Xiaoming;Qiao, Weitao;Gao, Dayong
玻璃化保存是通过避免细胞内冰形成来长期储存生物材料（例如细胞悬浮液）的一种有前景的方法，而超快速冷却是实现玻璃化的关键因素。在这项研究中，介绍并研究了一种新型冷却系统。物理过程，包括液氮在微通道中的流动沸腾以及系统中膜状样品溶液的冷却和凝固，均在理论上进行了建模。通过模拟样品溶液的局部冷却速率和结晶程度，评估系统的冷却性能。案例研究表明，此类系统有望实现样品溶液的超高冷却速率和高玻璃化趋势。此外，系统尺寸可以在灵活的范围内调整，以允许保存各种体积的样品。总之，新型冷却系统有望降低玻璃化冷冻所需的冷冻保护剂浓度，扩大玻璃化保存的应用领域，本研究提出的模型可以成为指导此类系统设计和应用的有用工具。 (C) 2014 Elsevier Ltd. 保留所有权利。
Vitrification preservation is a promising approach for long term storage of biomaterials (e.g. cell suspension) by avoiding intracellular ice formation, and ultra-fast cooling is the key factor to achieve vitrification. In this study, a novel cooling system is introduced and investigated. Physical processes, including flow boiling of liquid nitrogen in microchannels as well as cooling and solidification of the film-shaped sample solution in the system, are theoretically modeled. By simulating the local cooling rate and degree of crystallization in sample solution, the cooling performance of the system is evaluated. Case studies indicate ultra-high cooling rates and high vitrification tendency of sample solution are promisingly achieved with such system. Furthermore, the system dimensions can be adjusted in a flexible range to allow preserving of various volumes of samples. In conclusion, the novel cooling system will hopefully decrease the required concentration of cryoprotectant for vitrification and extend the application area of vitrification preservation, and the model presented in this study can be a useful tool to guide the design and application of such system. (C) 2014 Elsevier Ltd. All rights reserved.