Application of a novel method for phosphorus determinations in the oligotrophic North Pacific Ocean
Application of a novel method for phosphorus determinations in the oligotrophic North Pacific Ocean
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DOI：
10.4319/lo.1998.43.7.1565
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发表时间：
1998-11-01
影响因子：
4.5
通讯作者：
Karl, D
中科院分区：
文献类型：
--
作者：
Thomson-Bulldis, A;Karl, D
磷（P）是一种必需的常量营养素，以溶解的无机（主要是正磷酸盐，Pi）和有机化合物的形式存在于海水中。目前的分析技术测量可溶性活性磷（SRP）作为Pi的替代物，可溶性非活性磷作为溶解有机磷（DOP）的替代物。一种新的方法提供了一种直接测量的Pi浓度，并消除了其他反应性P化合物的干扰。同样的方法也可以用于直接测量非磷（N-PiP）浓度（即，[总溶解P] - [Pi]），具有比当前方法提供的更高的精密度和准确度限值。使用这种新方法，发现北太平洋表面沃茨中的Pi浓度比使用标准自动分析仪技术测量的SRP浓度低50%或更多。北太平洋深海（> 1，000 m）的平均（+/-SD）N-PiP浓度确定为16.2 +/- 12.5 nM。这些结果对溶解磷池的周转和停留时间以及DOP在深海中的利用具有重要意义。这种测量Pi和N-PiP的新方法在海洋和淡水研究中具有许多潜在的应用。
Phosphorus (P) is an essential macronutrient and is present in seawater as both dissolved inorganic (primarily orthophosphate, Pi) and organic compounds. Current analytical techniques measure soluble reactive phosphorus (SRP) as a surrogate for Pi and soluble nonreactive phosphorus as a surrogate for dissolved organic phosphorus (DOP). A new method provides a measure of Pi concentrations directly and eliminates interference from other reactive P compounds. The same method can also be used to make direct measurements of non-Pi phosphorus (N-PiP) concentrations (i.e., [total dissolved P] - [Pi]) with greater precision and accuracy limits than current methods provide. With this new method, Pi concentrations in North Pacific surface waters were found to be lower, by up to 50% or more, than SRP concentrations measured with standard autoanalyzer techniques. Mean (+/-SD) N-PiP concentrations in the deep (>1,000 m) North Pacific were determined to be 16.2 +/- 12.5 nM. These results have significant implications for turnover and residence times of dissolved P pools and for utilization of DOP in the deep sea. This new method for Pi and N-PiP measurements has numerous potential applications in marine and freshwater research.