Three-layer flow membrane system on a microchip for investigation of molecular transport.
Three-layer flow membrane system on a microchip for investigation of molecular transport.
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DOI：
10.1021/ac0112317
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发表时间：
2002-03
影响因子：
7.4
通讯作者：
M. Surmeian;M. Slyadnev;H. Hisamoto;A. Hibara;K. Uchiyama;T. Kitamori
中科院分区：
文献类型：
--
作者：
M. Surmeian;M. Slyadnev;H. Hisamoto;A. Hibara;K. Uchiyama;T. Kitamori
一个稳定的三层流动系统，水/有机溶剂/水，已被成功地应用于第一次在微通道中，以获得快速传输通过有机液膜。在连续层流区，分析物（甲基红）被快速提取通过微通道从供体到受体相通过有机溶剂相（环己烷）。热透镜显微镜用于监测该过程。夹在两个水相之间的有机相的厚度约为64微米，并且它被认为是薄的液体有机膜。渗透性的研究表明，分子扩散，层厚度，和有机溶剂-水分配系数的分子运输的影响。在微芯片中，完全平衡在几秒钟内实现，而传统使用的设备需要几十分钟。的有机和水的边界层的厚度被定义为等于微通道的尺寸，和有机溶剂-水的分配系数被确定在微芯片上使用的液/液萃取系统。有机膜分子传输的实验数据与基于三室水/有机溶剂/水模型计算的渗透率一致。这种实验只能在微空间中进行，该系统可以被认为是一种潜在的生物膜，为未来的药物转运的体外研究。
A stable three-layer flow system, water/organic solvent/water, has been successfully applied for the first time in a microchannel to get rapid transport through an organic liquid membrane. In the continuous laminar flow region, the analyte (methyl red) was rapidly extracted across the microchannel from the donor to the acceptor phase through the organic solvent phase (cyclohexane). Thermal lens microscopy was used to monitor the process. The thickness of the organic phase, sandwiched by the two aqueous phases, was approximately 64 microm, and it was considered as a thin liquid organic membrane. Permeability studies showed the effects of molecular diffusion, layer thickness, and organic solvent-water partition coefficient on the molecular transport. In the microchip, complete equilibration was achieved in several seconds, in contrast to a conventionally used apparatus, where it takes tens of minutes. The thickness of the organic and aqueous boundary layers was defined as equal to the microchannel dimensions, and the organic solvent-water partition coefficient was determined on a microchip using the liquid/liquid extraction system. Experimental data on molecular transport across the organic membrane were in agreement with the calculated permeability based on the three-compartment water/organic solvent/water model. This kind of experiment can be performed only in a microspace, and the system can be considered as a potential biological membrane for future in vitro study of drug transport.