Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films
Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films
复制标题
DOI：
10.1016/s0142-9612(99)00155-6
复制
发表时间：
1999-12-01
期刊：
影响因子：
14
通讯作者：
Ratcliffe, A
中科院分区：
文献类型：
--
作者：
Ishaug-Riley, SL;Okun, LE;Ratcliffe, A
在合成可降解聚合物膜和作为对照的组织培养聚苯乙烯 (TCPS) 上评估了聚合物化学对人类关节软骨 (HAC) 软骨细胞粘附、增殖和形态的影响。聚(乙交酯) (PGA)、聚(L-丙交酯) (L-PLA)、聚(D,L-丙交酯) (D,L-PLA)、85:15 聚(D,L-丙交酯-共-乙交酯) (D,L-PLGA)、聚(ε-己内酯) (PCL)、90:10（D,L-丙交酯-共-己内酯）的二维表面(D,L-PLCL)、9:91 D,L-PLCL、40:60 L-PLCL、67:33 聚乙交酯-三亚甲基碳酸酯 (PGTMC) 和聚二氧环己酮 (PDO) 通过旋涂制成均匀薄膜。使用 TCP 和 PCL 薄膜研究粘附动力学，结果表明软骨细胞粘附率在 6 小时后开始趋于平稳。 8 小时后，研究了所有基底上 HAC 软骨细胞粘附的程度，范围为 TCPS 上发现的粘附的 47% 至 145%。附着在 PGA 和 67:33 PGTMC 聚合物薄膜上的软骨细胞数量最多，附着在 PCL 和 L-PLA 薄膜上的软骨细胞数量统计上低于 PGA 上的软骨细胞 (p < 0.05)。软骨细胞附着于基底的量与基底的水接触角之间不存在相关性。软骨细胞在所有基质上增殖得同样好，导致培养第 4 天和第 7 天所有基质上的细胞数量相等。然而，这些总细胞数是通过 PDO 和 PLA 分别扩增 88 倍和 42 倍而达到的，明显高于 TCPS 上的 Ii 倍扩增 (p < 0.05)。 PDO 和 L-PLA 薄膜上细胞的更大倍数扩张可能归因于细胞生长的空间的可用性，因为在 8 小时贴壁期后，它们在培养开始时的数量较少。这表明，无论这些可降解聚合物基材上的初始接种密度如何（即，如果能够附着一些最小数量的细胞），只要有足够的空间和时间，它们最终将填充所有这些聚合物的表面。 (C) 1999 Elsevier Science Ltd. 保留所有权利。
The effect of polymer chemistry on adhesion, proliferation, and morphology of human articular cartilage (HAC) chondrocytes was evaluated on synthetic degradable polymer films and tissue culture polystyrene (TCPS) as a control. Two-dimensional surfaces of poly(glycolide) (PGA), poly(L-lactide) (L-PLA), poly(D,L-lactide) (D,L-PLA), 85:15 poly(D,L-lactide-co-glycolide) (D,L-PLGA), poly(epsilon-caprolactone) (PCL), 90:10 (D,L-lactide-co-caprolactone) (D,L-PLCL), 9:91 D,L-PLCL, 40:60 L-PLCL, 67:33 poly(glycolide-co-trimethylene carbonate) (PGTMC), and poly(dioxanone) (PDO) were made by spin-casting into uniform thin films. Adhesion kinetics were studied using TCPs and PCL films and revealed that the rate of chondrocyte adhesion began to level off after 6 h. Degree of HAC chondrocyte adhesion was studied on all the substrates after 8 h, and ranged from 47 to 145% of the attachment found on TCPS. The greatest number of chondrocytes attached to PGA and 67:33 PGTMC polymer films, and attachment to PCL and L-PLA films was statistically lower than that found on PGA (p < 0.05). There was no correlation between amount of chondrocyte attachment to the substrates and the substrates' water contact angle. Chondrocytes proliferated equally well on all the substrates resulting in equivalent cell numbers on all the substrates at both day 4 and day 7 of the culture. However, these total cell numbers were reached as a result of a 88- and 42-fold expansion on PDO and PLA, respectively, which was significantly higher than the Ii-fold expansion found on TCPS (p < 0.05). The greater fold expansion of the cells on PDO and L-PLA films may be attributed to the availability of space for cells to grow, since their numbers at the start of culture were fewer following the 8 h attachment period. This suggests that regardless of initial seeding density on these degradable polymer substrates (i.e., if some minimum number of cells are able to attach), they will eventually populate the surfaces of all these polymers given sufficient space and time. (C) 1999 Elsevier Science Ltd. All rights reserved.