Rapid regeneration of protochlorophyllide(650).
Rapid regeneration of protochlorophyllide(650).
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原叶绿素内酯(650)的快速再生。
DOI：
10.1104/pp.45.2.201
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发表时间：
1970
期刊：
影响因子：
7.4
通讯作者：
M. Gassman
中科院分区：
文献类型：
--
作者：
S. Granick;M. Gassman
使用 8 至 9 天的暗生豆叶进行饱和闪光后，通过分光光度法检查原脱氧核苷 (650) 的再生率。再生率达到 15%，半升时间约为 20 秒。在黑暗中向切下的叶子喂食 δ-氨基乙酰丙酸会增加原叶绿素内酯 (635)，但不会增加 650 纳米处的吸收，这表明全息色素通常被原叶绿素内酯饱和，并且全息色素蛋白质不受原叶绿素内酯水平控制。闪光后，由外源 δ-氨基乙酰丙酸形成的过量原叶绿素很容易结合以再生 650 纳米吸收物质；再生率达到 60% 至 80%，半升时间约为 50 秒。再生在 0 度被阻断，表明再生需要一些酶促过程，可能是原脱氧核苷 (650) 全色素的还原剂成分的形成。
The rate of regeneration of protochlorophyllide(650) was examined spectrophotometrically after a saturating light flash using 8- to 9-day-old dark-grown bean leaves. The regeneration occurred to the extent of 15% with a half rise time of about 20 seconds. Feeding delta-aminolevulinic acid to the excised leaves in the dark increased protochlorophyllides(635) but not the absorption at 650 nanometers, suggesting that the holochrome was normally saturated with protochlorophyllide and that the holochrome protein was not controlled by the level of protochlorophyllide. After a light flash, the excess protochlorophyllide, formed from exogenous delta-aminolevulinic acid, readily combined to regenerate the 650 nanometer absorbing species; the regeneration occurred to the extent of 60 to 80% with a half rise time of about 50 seconds. Regeneration was blocked at 0 degrees , suggesting that there was some enzymic process required for regeneration, possibly the formation of a reductant component of the protochlorophyllides(650) holochrome.