A diet rich in high-glucoraphanin broccoli interacts with genotype to reduce discordance in plasma metabolite profiles by modulating mitochondrial function
A diet rich in high-glucoraphanin broccoli interacts with genotype to reduce discordance in plasma metabolite profiles by modulating mitochondrial function
复制标题
DOI：
10.3945/ajcn.113.065235
复制
发表时间：
2013-09-01
影响因子：
7.1
通讯作者：
Mithen, Richard F.
中科院分区：
文献类型：
--
作者：
Armah, Charlotte N.;Traka, Maria H.;Mithen, Richard F.
背景资料：观察和实验研究表明，富含十字花科蔬菜和硫代葡萄糖苷的饮食可能会降低癌症和心血管疾病（CVD）的风险。目的：我们测试了一个假设，即与标准西兰花或豌豆相比，使用高硫代葡萄糖苷（HG）西兰花进行12周的饮食干预会在更大程度上改变CVD风险和血浆代谢产物谱的生物标志物。设计：受试者被随机分配到每周食用400 g标准西兰花，400 g HG西兰花或400 g豌豆，持续12周，没有其他饮食限制。CVD风险和347血浆代谢物的生物标志物进行了量化之前和之后intervention.Results：没有显着差异的影响，饮食对CVD风险的生物标志物被发现。血浆代谢物的多变量分析确定2离散的表型反应，饮食在个人内的HG西兰花臂，区分与PAPOLG基因的单核苷酸多态性。单因素分析显示性别（P < 0.001）、PAPOLG基因型（P < 0.001）和PAPOLG基因型X饮食（P < 0.001）对血浆代谢谱的影响。在HG西兰花臂，干预的后果是减少脂质和氨基酸代谢产物，三羧酸（TCA）循环中间体，和酰基肉毒碱之间的变化2 PAPOLG genotype.Conclusions：观察到的代谢变化与HG西兰花饮食是一致的再平衡的回补和cataplerotic反应和增强整合的脂肪酸β-氧化与TCA循环活性。这些改变可能有助于减少与富含十字花科蔬菜的饮食相关的癌症风险。本试验在clinicaltrials.gov上注册为NCT 01114399。
Background: Observational and experimental studies suggest that diets rich in cruciferous vegetables and glucosinolates may reduce the risk of cancer and cardiovascular disease (CVD).Objective: We tested the hypothesis that a 12-wk dietary intervention with high-glucoraphanin (HG) broccoli would modify biomarkers of CVD risk and plasma metabolite profiles to a greater extent than interventions with standard broccoli or peas.Design: Subjects were randomly assigned to consume 400 g standard broccoli, 400 g HG broccoli, or 400 g peas each week for 12 wk, with no other dietary restrictions. Biomarkers of CVD risk and 347 plasma metabolites were quantified before and after the intervention.Results: No significant differences in the effects of the diets on biomarkers of CVD risk were found. Multivariate analyses of plasma metabolites identified 2 discrete phenotypic responses to diet in individuals within the HG broccoli arm, differentiated by single nucleotide polymorphisms associated with the PAPOLG gene. Univariate analysis showed effects of sex (P < 0.001), PAPOLG genotype (P < 0.001), and PAPOLG genotype X diet (P < 0.001) on the plasma metabolic profile. In the HG broccoli arm, the consequence of the intervention was to reduce variation in lipid and amino acid metabolites, tricarboxylic acid (TCA) cycle intermediates, and acylcarnitines between the 2 PAPOLG genotypes.Conclusions: The metabolic changes observed with the HG broccoli diet are consistent with a rebalancing of anaplerotic and cataplerotic reactions and enhanced integration of fatty acid beta-oxidation with TCA cycle activity. These modifications may contribute to the reduction in cancer risk associated with diets that are rich in cruciferous vegetables. This trial was registered at clinicaltrials.gov as NCT01114399.