RNase HII from Chlamydia pneumoniae discriminates mismatches incorporation into DNA-rN1-DNA/DNA duplexes
RNase HII from Chlamydia pneumoniae discriminates mismatches incorporation into DNA-rN1-DNA/DNA duplexes
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DOI：
10.1016/j.bbrc.2007.03.075
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发表时间：
2007-05-18
影响因子：
3.1
通讯作者：
Liu, Jianhua
中科院分区：
文献类型：
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作者：
Hou, Jingli;Liu, Xipeng;Liu, Jianhua
肺炎衣原体核糖核酸酶H Ⅱ（CpRNase H Ⅱ）对RNA/DNA双链体具有核糖核酸酶H活性。我们分析了CpRNase HII对DNA-rN（1）-DNA/DNA双链体（rN（1），一个核糖核苷酸）的切割特异性。在DNA-rN（1）-DNA/DNA双链体的核糖核苷酸处或周围引入了各种错配。与匹配的双链体相比，双链体的错配导致较慢的切割速率。此外，对于位于核糖核苷酸处或邻近核糖核苷酸的错配，观察到切割活性的更大降低。DNA-rN（1）-DNA/DNA双链体的同一位置的错配对CpRNase HII的切割速率有不同的影响，这取决于错配的类型。这些发现可能提供进一步的见解CpRNase III-底物相互作用的物理结合和催化特性。(c)2007年爱思唯尔公司All rights reserved.
It was reported that RNase HII from Chlamydia pneumoniae (CpRNase HII) had RNase H activity on RNA/DNA duplex. We have analyzed the cleavage specificity of CpRNase HII on DNA-rN(1)-DNA/DNA duplex (rN(1), one ribonucleotide). Various mismatches were introduced into the DNA-rN(1)-DNA/DNA duplexes at or around the ribonucleotide. The mismatches of duplexes resulted in slower cleavage rates compared to the matched duplexes. Furthermore, a greater reduction in cleavage activity was observed for the mismatches located at or adjacent to the ribonucleotide. The mismatches at the same position of DNA-rN(1)-DNA/DNA duplexes have different impact on the cleavage rates of CpRNase HII depending on the types of mismatches. These findings may offer further insights into the physical binding and catalytic properties of CpRNase HII-substrate interaction. (c) 2007 Elsevier Inc. All rights reserved.