Effects of the plant flavonoids silymarin and quercetin on arsenite-induced oxidative stress in CHO-K1 cells
Effects of the plant flavonoids silymarin and quercetin on arsenite-induced oxidative stress in CHO-K1 cells
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DOI：
10.1016/j.fct.2006.12.002
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发表时间：
2007-06-01
影响因子：
4.3
通讯作者：
Eynard, A. R.
中科院分区：
文献类型：
--
作者：
Bongiovanni, G. A.;Soria, E. A.;Eynard, A. R.
饮用水中砷的慢性毒性影响是一个人类健康问题，特别是在南美洲和亚洲。砷能够影响各种细胞过程，造成不良影响，包括癌症。虽然确切的作用机制尚不清楚，但已观察到氧化应激、肿瘤促进和砷暴露之间的相关性。我们研究了水飞蓟素和槲皮素在对抗急性或亚慢性亚砷酸钠暴露产生的氧化应激中的作用。砷的应激反应包括热休克蛋白70 kDa的表达，脂质过氧化测定共轭二烯措施，和γ-谷氨酰转肽酶活性的增加。在急性实验中，我们发现所有这些应激反应都被水飞蓟素和槲皮素消除。这两种黄酮类化合物减少了共轭二烯的形成在亚慢性文化。我们的研究结果表明，这些抗氧化剂类黄酮，这可能是很容易纳入饮食，可以提供对亚砷酸盐诱导的细胞毒性的保护作用。(C)2006爱思唯尔有限公司保留所有权利。
Chronic toxic effects of arsenic resulting from drinking water are a human health problem, especially in South-America and Asia. Arsenic is capable of influencing various cellular processes, causing adverse effects, including cancer. Although the exact mechanism of the action is not known, a correlation between oxidative stress, tumour promotion and arsenic exposure has been observed. We examined the effects of silymarin and quercetin, in counteracting oxidative stress produced by acute or sub-chronic sodium arsenite exposure. The stress responses to arsenite included an increase in the heat shock protein 70 kDa expression, lipid peroxidation assayed by conjugated dienes measure, and gamma-glutamyl-transpepticlase activity. We found that all these stress responses were eliminated by silymarin and quercetin in acute experiments. Both flavonoids diminished the conjugated dienes formation during sub-chronic cultures. Our results suggest that these antioxidant flavonoids, which may be easily incorporated into the diet, may afford a protective effect against arsenite-induced cytotoxicity. (C) 2006 Elsevier Ltd. All rights reserved.