Dbp6p is an essential putative ATP-dependent RNA helicase required for SOS-ribosomal-subunit assembly in Saccharomyces cerevisiae
Dbp6p is an essential putative ATP-dependent RNA helicase required for SOS-ribosomal-subunit assembly in Saccharomyces cerevisiae
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DOI：
10.1128/mcb.18.4.1855
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发表时间：
1998-04-01
影响因子：
5.3
通讯作者：
Linder, P
中科院分区：
文献类型：
--
作者：
Kressler, D;De la Cruz, J;Linder, P
在欧洲功能分析网络的背景下，以前未表征的酿酒酵母开放阅读框架，YNR 038 W，进行了分析。YNR 038 W编码DEAD-盒蛋白家族的一种推定的ATP依赖性RNA解旋酶，因此被命名为DBP 6（DEAD-盒蛋白6）。Dbp 6 p对于细胞活力至关重要。体内Dbp 6 p的缺失导致60 S核糖体亚基的缺陷和半聚体多聚体的出现。用脉冲追踪法标记前体rRNA，用北方杂交和引物延伸法对前体rRNA和成熟rRNA进行稳态分析，结果表明Dbp 6p缺失导致27 S和7S前体的产生减少，导致成熟的25 S和5.8S rRNA缺失。此外，血凝素表位标记的Dbp 6p仅在核仁内检测到。我们建议，Dbp 6p是需要适当的装配preribosomal颗粒在60 S核糖体亚基的生物发生过程中，可能是作为一个rRNA解旋酶。
A previously uncharacterized Saccharomyces cerevisiae open reading frame, YNR038W, was analyzed in the context of the European Functional Analysis Network. YNR038W encodes a putative ATP-dependent RNA helicase of the DEAD-box protein family and was therefore named DBP6 (DEAD-box protein 6). Dbp6p is essential for cell viability. In vivo depletion of Dbp6p results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. Pulse-chase labeling of pre-rRNA and steady-state analysis of pre-rRNA and mature rRNA by Northern hybridization and primer extension show that Dbp6p depletion leads to decreased production of the 27S and 7S precursors, resulting In a depletion ok the mature 25S and 5.8S rRNAs. Furthermore, hemagglutinin epitope-tagged Dbp6p is detected exclusively within the nucleolus. We propose that Dbp6p is required for the proper assembly of preribosomal particles during the biogenesis of 60S ribosomal subunits, probably by acting as an rRNA helicase.