COMPARATIVE VIRULENCE OF INTRASTRAIN AND INTERSTRAIN LIPOPOLYSACCHARIDE VARIANTS OF COXIELLA-BURNETII IN THE GUINEA-PIG MODEL
COMPARATIVE VIRULENCE OF INTRASTRAIN AND INTERSTRAIN LIPOPOLYSACCHARIDE VARIANTS OF COXIELLA-BURNETII IN THE GUINEA-PIG MODEL
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DOI：
10.1128/iai.55.5.1144-1150.1987
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发表时间：
1987-05-01
影响因子：
3.1
通讯作者：
HACKSTADT, T
中科院分区：
文献类型：
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作者：
MOOS, A;HACKSTADT, T
我们比较了贝氏柯克斯体九里菌株与心内膜炎型菌株代表Priscilla菌株的脂多糖（LPS）变体的相对感染性和毒力。与先前的研究结果一致，九里I期（9 mi/I）微生物具有高度感染性，在接种物中含有少至4种微生物时引起血清转化和发热。感染后30天，从感染动物的脾脏中回收到活的9 ml/l。九英里II期（9 mi/II）微生物未引起发热或血清转化，除非接种量非常大，并且在感染后30天无法回收活微生物。Nine Mile/Crazy变异株携带中间型LPS，通过发热反应和血清转化测定，也具有高度感染性，尽管与9 mi/II一样，感染后30天无法恢复活菌。根据感染后30天的血清转化及其在脾脏中的存在，I期Priscilla菌株（Pris/I）的感染性为9 mi/I;但与9 mi/I相反，需要超过10（5）个Pris/I分离株才能诱导发热。两种菌株的抗I相和II相抗体的时间外观相似。在凝集、免疫荧光、酶联免疫吸附和免疫印迹试验中，多种血清学技术测量针对全细胞和纯化LPS抗原的抗体应答，但未表现出足够的特异性来区分9 mi/I和Pris/I感染。疫苗交叉攻毒实验结果表明，同源和异源攻毒菌株之间的保护程度显着。同源保护
We compared the relative infectivity and virulence of lipopolysaccharide (LPS) variants of the Nine Mile strain of Coxiella burnetii with those of the Priscilla strain, a representative of endocarditis-type strains. In agreement with results of previous studies, Nine Mile phase I (9mi/I) organisms were highly infectious, eliciting seroconversion and fever with inocula containing as few as four organisms. Viable 9mi/I was recovered from the spleens of infected animals 30 days postinfection. Nine Mile phase II (9mi/II) organisms did not elicit fever or seroconversion except with very large inocula, and viable organisms could not be recovered at 30 days postinfection. The Nine Mile/Crazy variant, bearing the intermediate-type LPS, was also highly infectious, as determined by fever response and seroconversion, although, as with 9mi/II, viable organisms could not be recovered 30 days postinfection. The Priscilla strain in phase I (Pris/I) was as infectious as 9mi/I, as determined by seroconversion and its presence in the spleen 30 days postinfection; but in contrast to 9mi/I, more than 10(5) Pris/I isolates were required to induce fever. The temporal appearances of anti-phase I and II antibodies were similar for the two strains. A variety of serological techniques measuring antibody response against whole-cell and purified LPS antigens in agglutination, immunofluorescence, enzyme-linked immunosorbent, and immunoblot assays did not demonstrate sufficient specificity to distinguish between 9mi/I and Pris/I infections. Results of vaccine cross-challenge experiments showed a significant degree of protection between homologous and heterologous challenge strains. protection between homologous