Clonorchis sinensis:: Development and evaluation of a nested polymerase chain reaction (PCR) assay
Clonorchis sinensis:: Development and evaluation of a nested polymerase chain reaction (PCR) assay
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DOI：
10.1016/j.exppara.2006.09.010
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发表时间：
2007-03-01
影响因子：
2.1
通讯作者：
Karunasagar, Iddya
中科院分区：
文献类型：
--
作者：
Parvathi, Ammini;Kumar, H. Sanath;Karunasagar, Iddya
华支睾吸虫是一种东亚特有的鱼媒吸虫，全球有3500多万人感染。在本文的研究中，我们建立了一种巢式聚合酶链反应（PCR）方法，用于特异性和可靠地检测中华绒螯蟹。根据本研究获得的核苷酸序列数据设计引物，对其检测中华绒螯蟹的特异性和敏感性进行了评价。特异性扩增产物仅在中华绒螯蟹中获得，而与之亲缘关系较近的吸虫（包括蛇胸虫）DNA均未扩增出特异性扩增产物，证明了该方法的特异性。本文所描述的新型PCR方法将有助于鱼产品的检疫和华支睾吸虫病在疫区传播状况的评估。(c) 2006爱思唯尔公司版权所有。
Clonorchis sinensis is a fish-borne trematode endemic to East Asia, which infects over 35 million people globally. In the study described here, we developed a nested polymerase chain reaction (PCR) method for the specific and reliable detection of C sinensis. The primers designed from the nucleotide sequence data derived in this study were evaluated for their specificity and sensitivity for the detection of C sinensis. The specific amplification products were obtained only with C sinensis and no amplifications occurred with the DNA of closely related trematodes including Opisthorchis viverrini demonstrating the specificity of the assay. The novel PCR method described here will be useful for the quarantine of fishery products and evaluation of transmission status of clonorchiasis in the endemic areas. (c) 2006 Elsevier Inc. All rights reserved.