Method development for thermal ionization mass spectrometry in the frame of a biokinetic tracer study with enriched stable isotopes of zirconium
Method development for thermal ionization mass spectrometry in the frame of a biokinetic tracer study with enriched stable isotopes of zirconium
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DOI：
10.1016/j.ijms.2011.02.013
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发表时间：
2011-06-15
影响因子：
1.8
通讯作者：
Oeh, Uwe
中科院分区：
文献类型：
--
作者：
Greiter, Matthias B.;Hoellriegl, Vera;Oeh, Uwe
热电离质谱中的同位素稀释 (ID-TIMS) 是一种用于定量地质样品中同位素比率或用于地质和环境样品中痕量元素分析的精确方法。这项工作提出了一种优化的 ID-TIMS 方法，适用于生命科学领域，更准确地说，是在通过口服和静脉注射两种稳定同位素示踪剂对锆进行人体生物动力学研究的框架中。该方法可以同时分析人体血浆和尿液中不同的稳定锆同位素（两种同位素富集的人工示踪剂和自然背景）。通过优化，可以实现低于 1 ng ml(-1) 的示踪剂检测限。生物样品通过微波辅助酸性压力消化和萃取色谱法制备。在碳涂层铼单丝上测量样品中纯化的锆。使用多通道电子倍增器作为检测器。观察到钼同位素的干扰，并根据 Mo-95 计数率进行校正。锆的同位素比测定范围为0.016-2.84（Zr-91/Zr-90）、0.020-2.46（Zr-92/Zr-90）、0.115-10.93（Zr-94/Zr-90）和0.004-5.70（Zr-96/Zr-90）。各自的相对不确定性在0.03-4.2%范围内。示踪剂浓度的典型相对不确定度为 4%。 (C) 2011 Elsevier B.V. 保留所有权利。
Isotope dilution in thermal ionization mass spectrometry (ID-TIMS) is a precise method for quantification of isotope ratios in geological samples, or for trace elements analysis in geological and environmental samples. This work presents an optimized ID-TIMS method for application in the field of life sciences, more precisely in the frame of a human biokinetic study on zirconium with oral and intravenous administration of two stable isotope tracers. The method allows analyzing simultaneously different stable zirconium isotopes (two isotopically enriched artificial tracers and the natural background) in human blood plasma and urine. By optimization, tracer detection limits below 1 ng ml(-1) could be achieved. The biological samples were prepared by microwave-assisted acidic pressure digestion followed by extraction chromatography. The purified zirconium from the samples was measured on carbon-coated rhenium single filaments. Multiple channel electron multipliers were used as detectors. Interferences were observed from molybdenum isotopes and were corrected for based on the Mo-95 count rate. Isotope ratios of zirconium were determined in ranges of 0.016-2.84 (Zr-91/Zr-90), 0.020-2.46 (Zr-92/Zr-90), 0.115-10.93 (Zr-94/Zr-90), and 0.004-5.70 (Zr-96/Zr-90). The respective relative uncertainties lay in the range of 0.03-4.2%. Typical relative uncertainties of tracer concentrations were 4%. (C) 2011 Elsevier B.V. All rights reserved.