Ultrasensitive Sensor Using Quantum Dots-Doped Polystyrene Nanospheres for Clinical Diagnostics of Low-Volume Serum Samples
Ultrasensitive Sensor Using Quantum Dots-Doped Polystyrene Nanospheres for Clinical Diagnostics of Low-Volume Serum Samples
复制标题
使用量子点掺杂聚苯乙烯纳米球的超灵敏传感器用于低容量血清样本的临床诊断
DOI：
10.1021/acs.analchem.9b00010
复制
发表时间：
2019-05-07
影响因子：
7.4
通讯作者：
Wu, Yingsong
中科院分区：
文献类型：
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作者：
Chen, Zhenhua;Li, Peng;Wu, Yingsong
开发灵敏的均相测定是临床诊断的高优先级研究目标。量子点具有良好的物理化学性质，这意味着它们在荧光检测中具有潜在的优势。基于量子点的生物传感器已经在文献中描述;然而，它们中很少有真正发展到广泛的临床应用。在这项工作中，荧光均相检测生物传感器的制备使用量子点掺杂的聚苯乙烯纳米球，灵敏地检测小体积血清样品中的生物标志物。制备了酞菁染色和量子点封装的表面带有羧基的羧基功能化聚苯乙烯纳米球（PPs和QPs），并分别作为触发剂和荧光探针应用于该生物传感系统。在这种双波长形式的免疫测定中，PP一旦被680 nm二极管激光器敏化就产生单线态氧，并且与抗体缀合的QP然后在特异性抗原存在下与单线态氧反应，并且由于QP内的荧光共振能量转移（FRET）而发射波长约为605 nm的反斯托克斯荧光。我们证明了在25 μ L的血清样品中癌胚抗原作为一个模型蛋白质的目标与前所未有的检测限为2.56 × 10（-13）M（46 pg/mL）使用该生物传感器的测定。与市售化学发光免疫分析试剂盒的相关性良好（R-2 = 0.99718，n = 107）。这些结果表明，我们的灵活和可靠的生物传感器是适合直接集成到临床诊断，它有望成为一个有前途的诊断工具，早期检测和筛选测试以及预后评估的患者。
Development of sensitive homogeneous assays is a high-priority research target for clinical diagnostics. Quantum dots (QDs) present favorable photophysical properties, which implies their potential as an exceptional dye in fluorescence detection. QDs-based biosensors have been described in the literature; however, few of them have truly progressed to widespread clinical usage. In this work, a chemiluminescent homogeneous detecting biosensor is fabricated using QDs-doped polystyrene nanospheres to sensitively detect biomarkers in low-volume serum samples. Phthalocyanine-dyed and QDs-encapsulated carboxylate-functionalized polystyrene nanospheres with surface carboxyl groups (PPs and QPs, respectively) were fabricated and served as triggers and fluorescent probes, respectively, in this biosensing system. In this sandwich-format immunoassay, the PPs produced singlet oxygen once sensitized by 680 nm diode lasers, and the QPs, conjugated with antibodies, and then reacted with the singlet oxygen in the presence of specific antigens and emitted anti-Stokes fluorescence with wavelengths around 605 nm, as a result of fluorescence resonance energy transfer (FRET) within the QPs. We demonstrated the determination of carcinoembryonic antigen as a model protein target in 25 mu L of serum samples with an unprecedented detection limit of 2.56 X 10(-13) M (46 pg/mL) using this biosensor. Furthermore, excellent correlations (R-2 = 0.99718, n = 107) were obtained between utilizing this biosensor and commercialized chemiluminescence immunoassay kits in clinical serum detection. These results demonstrate that our flexible and reliable biosensor is suitable for direct integration into clinical diagnostics, and it is expected to be a promising diagnostic tool for early detection and screening tests as well as prognosis evaluation for patients.