Selective Coexpression of Multiple Chemical Markers Defines Discrete Populations of Neocortical GABAergic Neurons
Selective Coexpression of Multiple Chemical Markers Defines Discrete Populations of Neocortical GABAergic Neurons
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DOI：
10.1093/cercor/bhq252
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发表时间：
2011-08-01
期刊：
影响因子：
3.7
通讯作者：
Kawaguchi, Yasuo
中科院分区：
文献类型：
--
作者：
Kubota, Yoshiyuki;Shigematsu, Naoki;Kawaguchi, Yasuo
新皮质γ-氨基丁酸（GABA）细胞是否由有限数量的不同类别的神经元组成，或者它们是否以更高的多样性连续分化，仍然是该领域有争议的问题。大鼠额叶皮质的大多数GABA细胞具有6种化学标记物（小清蛋白、钙视网膜蛋白、α-辅肌动蛋白-2、生长抑素、血管活性肠多肽和胆囊收缩素）中的至少一种，每种化学类别包括具有特定生理和形态特征的几种不同的神经元亚型。为了更好地阐明GABA能神经元的多样性，我们评估了这6种化学标记物与促肾上腺皮质激素释放因子（CRF）、神经肽Y（NPY）、P物质受体（SPR）和一氧化氮合酶（NOS）的共定位;这4种额外的化学标记物表明在皮质GABA细胞中表达差异或特异性。我们进一步关联的形态学和生理学特征的一些化学亚类的抑制性神经元。我们的研究结果揭示了CRF，NPY，SPR和NOS在形态和生理上不同的中间神经元类的表达特异性。这些观察结果支持新皮质中存在有限数量的功能不同的GABA细胞亚型。
Whether neocortical gamma-aminobutyric acid (GABA) cells are composed of a limited number of distinct classes of neuron, or whether they are continuously differentiated with much higher diversity, remains a contentious issue for the field. Most GABA cells of rat frontal cortex have at least 1 of 6 chemical markers (parvalbumin, calretinin, alpha-actinin-2, somatostatin, vasoactive intestinal polypeptide, and cholecystokinin), with each chemical class comprising several distinct neuronal subtypes having specific physiological and morphological characteristics. To better clarify GABAergic neuron diversity, we assessed the colocalization of these 6 chemical markers with corticotropin-releasing factor (CRF), neuropeptide Y (NPY), the substance P receptor (SPR), and nitric oxide synthase (NOS); these 4 additional chemical markers suggested to be expressed diversely or specifically among cortical GABA cells. We further correlated morphological and physiological characteristics of identified some chemical subclasses of inhibitory neurons. Our results reveal expression specificity of CRF, NPY, SPR, and NOS in morphologically and physiologically distinct interneuron classes. These observations support the existence of a limited number of functionally distinct subtypes of GABA cells in the neocortex.