Noninvasive indexes of cardiac systolic and diastolic function in hyperthyroid and senescent mouse.
Noninvasive indexes of cardiac systolic and diastolic function in hyperthyroid and senescent mouse.
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DOI：
10.1152/ajpheart.1996.270.6.h2204
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发表时间：
1996-06
期刊：
影响因子：
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通讯作者：
G. Taffet;C. Hartley;Xiouying Wen;T. Pham;L. Michael;M. Entman
中科院分区：
文献类型：
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作者：
G. Taffet;C. Hartley;Xiouying Wen;T. Pham;L. Michael;M. Entman
小鼠是转基因操作的常用模型，然而，它们的小尺寸使得血流动力学研究困难。用无创性10 MHz脉冲多普勒探头测量麻醉、正常小鼠主动脉和二尖瓣血流速度，研究衰老和甲状腺素诱发的甲状腺功能亢进对心脏收缩和舒张功能的影响。在10只甲亢小鼠中，主动脉峰值速度（PAV，收缩功能指数）比10只对照小鼠高34%（108 +/- 2 vs. 80 +/- 3 cm/s，P < 0.05）。甲状腺功能亢进小鼠的二尖瓣早期充盈速度与晚期充盈速度之比（E/A比，舒张功能指数）高47%（5.6 +/- 0.8 vs. 3.8 +/- 0.2，P < 0.05）。在6只老年（30月龄）小鼠中，PAV与8只年轻（4月龄）小鼠相似（73 +/- 3 vs. 75 +/- 3 cm/s），但E/A比低59%（1.8 +/- 0.3 vs. 4.4 +/- 0.4，P < 0.05）。尽管观察到的心率范围很广，但各组的收缩和舒张参数明显分离。我们的结论是，心脏收缩和舒张功能的小鼠，脉冲多普勒超声测量，是类似于较大的物种，无论是在幅度和甲状腺功能亢进症和衰老的反应。
The mouse is a common model for transgenic manipulation, however, their small size has made hemodynamic study difficult. A noninvasive 10-MHz pulsed Doppler probe was used to measure aortic and mitral flow velocities in anesthetized, intact mice to study the effects of aging and hypethyroidism (induced by thyroxine) one systolic and diastolic cardiac function. In 10 hyperthyroid mice peak aortic velocity (PAV, an index of systolic function) was 34% higher than in 10 control mice (108 +/- 2 vs. 80 +/- 3 cm/s, P < 0.05). The ratio of early to late mitral filling velocity (E/A ratio, an index of diastolic function) was 47% higher (5.6 +/- 0.8 vs. 3.8 +/- 0.2, P < 0.05) in the hyperthyroid mice. In six old (30 mo) mice PAV was similar to eight young (4 mo) mice (73 +/- 3 vs. 75 +/- 3 cm/s), but the E/A ratio was 59% lower (1.8 +/- 0.3 vs. 4.4 +/- 0.4, P < 0.05). Despite a wide range of observed heart rates, the systolic and diastolic parameters of the groups were clearly separated. We conclude that cardiac systolic and diastolic function in mice, measured by pulsed Doppler ultrasound, are similar to larger species both in magnitude and in their response to hyperthyroidism and aging.