Gamma ray-induced bystander effect in tumour glioblastoma cells: A specific study on cell survival, cytokine release and cytokine receptors
Gamma ray-induced bystander effect in tumour glioblastoma cells: A specific study on cell survival, cytokine release and cytokine receptors
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DOI：
10.1093/rpd/ncl431
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发表时间：
2006-12-01
影响因子：
1
通讯作者：
Zilio, C.
中科院分区：
文献类型：
--
作者：
Facoetti, A.;Ballarini, F.;Zilio, C.
最近的实验证据已经挑战了辐射穿过细胞核是产生遗传变化或生物反应的先决条件的范式。因此，在直接照射的细胞或来自照射培养物的培养基的受体细胞附近的未暴露细胞也可能受到影响。本研究的目的是评估，通过介质转移技术，是否白细胞介素-8及其受体（CXCR 1）可能发挥作用的旁观者效应后，γ射线照射的T98 G细胞在体外。事实上，已经描述的诱导旁观者效应和接收分泌信号的细胞特异性表明，不仅释放细胞因子的能力，而且受体谱也可能调节细胞应答和最终结果。使用酶联免疫吸附测定定量的细胞因子释放到培养基中的剂量和时间依赖性表明，辐射导致暴露细胞中白细胞介素-8的释放以剂量非依赖性但时间依赖性的方式改变。在暴露和旁观者细胞中，相对受体表达也受到影响。
Recent experimental evidence has challenged the paradigm according to which radiation traversal through the nucleus of a cell is a prerequisite for producing genetic changes or biological responses. Thus, unexposed cells in the vicinity of directly irradiated cells or recipient cells of medium from irradiated cultures can also be affected. The aim of the present study was to evaluate, by means of the medium transfer technique, whether interleukin-8 and its receptor (CXCR1) may play a role in the bystander effect after gamma irradiation of T98G cells in vitro. In fact the cell specificity in inducing the bystander effect and in receiving the secreted signals that has been described suggests that not only the ability to release the cytokines but also the receptor profiles are likely to modulate the cell responses and the final outcome. The dose and time dependence of the cytokine release into the medium, quantified using an enzyme linked immnosorbent assay, showed that radiation causes alteration in the release of interleukin-8 from exposed cells in a dose-independent but time-dependent manner. The relative receptor expression was also affected in exposed and bystander cells.