Intraoral grafting of an ex vivo produced oral mucosa equivalent:: a preliminary report
Intraoral grafting of an ex vivo produced oral mucosa equivalent:: a preliminary report
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DOI：
10.1054/ijom.2002.0365
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发表时间：
2003-04-01
影响因子：
2.4
通讯作者：
Yoshizawa, M
中科院分区：
文献类型：
--
作者：
Izumi, K;Feinberg, SE;Yoshizawa, M
本研究的目的是评估使用离体产生的口腔粘膜等效物（EVPOME）进行口内移植手术的有效性。从手术前4周的穿刺活检中收获自体角质形成细胞。置于无血清培养系统中并接种到人尸体皮肤等同物AlloDerm上。根据疾病的阶段，将30名患有癌前病变或癌性病变的患者分为两组：第1组：EVPOME或第2组：AlloDerm（R），没有上皮层的对照。临床上，EVPOME移植物易于操作，并显示出良好的移植顺应性。EVPOME和AlloDerm移植物均显示出100%的摄取率。在移植后6天，EVPOME临床上显示出指示血管向内生长的变化，并且具有移植的培养的角质形成细胞在表面上持续存在的细胞学证据。在移植后28天进行的活组织检查中，与AlloDerm（R）移植物相比，EVPOME移植物具有与快速上皮覆盖相关的下层粘膜下层的增强的成熟。总之，EVPOME似乎是人口内移植手术的可接受的口腔粘膜替代物，并且比单独的AlloDerm（R）产生更有利的伤口愈合反应。
The objective of this study was to assess the efficacy of the use of an ex vivo produced oral mucosa equivalent (EVPOME) for intraoral grafting procedures. Autogenous keratinocytes were harvested from a punch biopsy 4 weeks phor to surgery. placed in a serum-free culture system and seeded onto a human cadaveric dermal equivalent, AlloDerm (R). Thirty patients with either a premalignant or cancerous lesion were triaged into two groups, depending on the stage of disease: Group 1: EVPOME or Group 2: AlloDerm (R), control without an epithelial layer. Clinically, EVPOME grafts were easy to handle and showed excellent compliance on grafting. Both, EVPOME and AlloDerm (R) grafts, showed a 100% take rate, At 6 days post-grafting, the EVPOME clinically showed changes indicating vascular ingrowth and had cytologic evidence of the persistence of grafted cultured keratinocytes on the surface. The EVPOME grafts had enhanced maturation of the underlying submucosal layer associated with rapid epithelial coverage when compared to the AlloDerm (R) grafts at biopsies taken at 28 days post-grafting. In summary, EVPOME appears to be an acceptable oral mucosal substitute for human intraoral grafting procedures and results in a more favorable wound healing response than AlloDerm (R) alone.