The effect of dynamic compression on the response of articular cartilage to insulin-like growth factor-I
The effect of dynamic compression on the response of articular cartilage to insulin-like growth factor-I
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DOI：
10.1016/s0736-0266(00)00004-8
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发表时间：
2001-01-01
影响因子：
2.8
通讯作者：
Trippel, SB
中科院分区：
文献类型：
--
作者：
Bonassar, LJ;Grodzinsky, AJ;Trippel, SB
关节软骨经常受到机械应力和细胞调节分子的影响。以前的研究表明，机械刺激可以影响关节软骨细胞的代谢活性，生物化学研究表明，生长因子和细胞因子控制许多相同的细胞功能。然而，很少有人知道的关系或相互作用，如果有的话，这两个关键组成部分的关节环境。本研究调查了低振幅，正弦的比较和相互作用的影响。动态压缩：和胰岛素样生长因子-I（IGF-I），滑液中的一种多肽，对软骨具有合成代谢作用。在牛髌股软骨外植体中，IGF-I使蛋白质和蛋白聚糖的合成增加了90%和120%。而动态压缩使蛋白质和蛋白聚糖的合成分别增加40%和90%。刺激IC;FI是显着大于动态压缩蛋白质和蛋白多糖的合成。当一起应用时，这两种刺激增强蛋白质和蛋白聚糖的合成分别为180%和290%，比单独使用任何一种刺激所达到的程度更大。IGF-I促进蛋白质合成的时间常数为12.2 h。动态压缩增加蛋白质合成的时间常数为2.9小时，速度显着快于IGF-I，这表明这些信号通过不同的细胞激活途径。当一起使用时，动态压缩和IGF-I以5.6 h的时间常数起作用。因此，动态压缩加速了对IGF-I的生物合成反应，并增加了IGF-I向关节软骨基质的转运，这表明，除了独立刺激关节软骨细胞外，循环压缩还可以改善可溶性生长因子进入这些相对孤立的细胞的途径。(C)2001骨科研究学会。由爱思唯尔科技有限公司出版。保留所有权利。
Articular cartilage is routinely subjected to mechanical farces and to cell-regulatory molecules, Previous studies have shown that mechanical stimuli can influence articular chondrocyte metabolic activity, and biochemical studies have shown that growth factors and cytokines control many of the same cell functions. Little is known, however, of the relationships or interplay, if any, between these two key components of the articular environment. This study investigated the comparative and interactive effects of low amplitude, sinusoidal. dynamic compression: and insulin-like growth factor-I (IGF-I) a polypeptide in synovial fluid that is anabolic for cartilage. In bovine patellofemoral cartilage explants, IGF-I increased protein and proteoglycan synthesis 90%, and 120%. respectively while dynamic compression increased protein and proteoglycan synthesis 40% and 90%, respectively. Stimulation by IC;FI was significantly greater than by dynamic compression for both protein and proteoglycan synthesis. When applied together, the two stimuli enhanced protein and proteoglycan synthesis by 180% and 290%, respectively, a degree greater than that achieved by either stimulus alone. IGF-I augmented protein synthesis with a time constant of 12.2 h. Dynamic compression increased protein synthesis with a time constant of 2.9 h, a rate significantly faster than that of IGF-I, suggesting that these signals act via distinct cell activation pathways. When used together, dynamic compression and IGF-I acted with a time constant of 5.6 h. Thus, dynamic compression accelerated the biosynthetic response to IGF-I and increased transport of IGF-I into the articular cartilage matrix, suggesting that, in addition to independently stimulating articular chondrocytes, cyclic compression may improve the access of soluble growth factors to these relatively isolated cells. (C) 2001 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.