Cellulase-producing bacteria of Aeromonas are dominant and indigenous in the gut of Ctenopharyngodon idellus (Valenciennes)
Cellulase-producing bacteria of Aeromonas are dominant and indigenous in the gut of Ctenopharyngodon idellus (Valenciennes)
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产纤维素酶的气单胞菌是草鱼 (Ctenopharyngodon idellus) (Valenciennes) 肠道中的优势菌和本土菌
DOI：
10.1111/j.1365-2109.2010.02645.x
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发表时间：
2011-03-01
影响因子：
2
通讯作者：
Bao, Baolong
中科院分区：
文献类型：
--
作者：
Jiang, Yan;Xie, Caixia;Bao, Baolong
草食性草鱼（Ctenopharyngodon idella）对水草有较强的消化能力。在草鱼肠道中发现有纤维素酶活性或产纤维素酶细菌。然而，仍然不确定产生纤维素酶的细菌是否是鱼所携带或随食物引入的固有肠道微生物群的一部分。本研究采用16SrRNA基因文库测序的方法，对饥饿草鱼肠道细菌的多样性和种群丰度进行了研究。16SrRNA基因文库分析表明，28株肠道寄生菌分别隶属于弧菌属、不动杆菌属、普罗威登斯菌属、耶尔森氏菌属、假单胞菌属、摩根氏菌属和气单胞菌属7个属，其中气单胞菌属是C. idella。所有分离自C. idella属于气单胞菌属。总体而言，本研究结果表明，C. idella应至少部分由产纤维素酶的气单胞菌属细菌引起，气单胞菌属细菌是本地和优势肠道菌种。
Herbivorous grass carp (Ctenopharyngodon idella) has a powerful capability to digest aquatic weed. Cellulase activity or cellulase-producing bacteria were found in the gut of grass carp. However, it remains uncertain whether the cellulase-producing bacteria were a part of indigenous intestinal microbiota that the fish harboured or were introduced with food. In the present study, the bacterial diversities and population abundance in the gut of starved grass carps have been investigated by sequencing 16S rRNA gene libraries. The 16S rRNA gene libraries revealed that 28 parasitic bacteria from gut were affiliated to seven genera of Vibrio, Acinetobacter, Providencia, Yersinia, Pseudominas, Morganella or Aeromonas, respectively, and Aeromonas was identified as the most dominant genus in the gut of C. idella. All of cellulase-producing bacteria isolated from the gut of C. idella in this research belonged to Aeromonas. On the whole, the results in this research showed that cellulase activity within C. idella should be at least partially resulting from bacteria of Aeromonas with cellulase-producing capabilities, which were indigenous and dominant intestinal species.