Maxillary sinus augmentation with Bio-Oss® particles:: A light, scanning, and transmission electron microscopy study in man
Maxillary sinus augmentation with Bio-Oss® particles:: A light, scanning, and transmission electron microscopy study in man
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DOI：
10.1002/jbm.b.30196
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发表时间：
2005-07-01
影响因子：
3.4
通讯作者：
Piattelli, A
中科院分区：
文献类型：
--
作者：
Orsini, G;Traini, T;Piattelli, A
骨-生物材料界面发生的生物相互作用对于长期临床成功至关重要。Bio-Oss（R）是一种脱蛋白、灭菌的牛骨，已广泛用于骨再生手术。本研究的目的是对鼻窦提升手术后取出的标本中Bio-Oss（R）和骨之间的界面进行比较性光学、扫描和电子显微镜评价。在光学显微镜下，大多数颗粒被新形成的骨包围，而在少数情况下，在一些颗粒的界面处，可以观察到骨髓腔和生物液体。在扫描电子显微镜下，在大多数情况下，颗粒周边出现内衬骨紧密粘附到生物材料表面。透射电子显微镜显示，生物材料周围的骨组织显示了骨愈合过程的所有阶段。在某些区域，存在随机组织的胶原纤维，而在其他区域，存在新形成的密质骨。在接触Bio-Oss（R）表面的第一个骨板中，胶原纤维的取向为243.73 +/- 7.12度（平均值+/- SD），而在其余骨板中，胶原纤维的取向为288.05 +/- 4.86度（平均值+/- SD），统计学显著差异为44.32度（p < 0.001）。在相同区域，生物材料表面附近的灰度值强度为172.56 +/- 18.15（平均值+/- SD），板层其他部分的灰度值强度为158.71 +/- 21.95（平均值+/- SD），差异不具有统计学意义，为13.79（P = 0.071）。在骨-生物材料界面处也有类似于骨水泥线的电子致密层。该层具有可变的形态，在一些区域中是细线，而在其他区域中是厚的不规则带。分析结果表明，Bio-Oss（R）颗粒不会干扰鼻窦提升手术后的正常骨愈合过程，并促进新骨形成。总之，本研究有助于更好地了解Bio-Oss（R）的形态学特征及其与周围组织的相互作用。(c)2005 Wiley Periodicals，Inc.
Biological interactions occurring at the bone-biomaterial interface are critical for long-term clinical success. Bio-Oss (R) is a deproteinized, sterilized bovine bone that has been extensively used in bone regeneration procedures. The aim of the present study was a comparative light, scanning, and electron microscopy evaluation of the interface between Bio-Oss (R) and bone in specimens retrieved after sinus augmentation procedures. Under light microscopy, most of the particles were surrounded by newly formed bone, while in a few cases, at the interface of some particles it was possible to observe marrow spaces and biological fluids. Under scanning electron microscopy, in most cases, the particle perimeter appeared lined by bone that was tightly adherent to the biomaterial surface. Transmission electron microscopy showed that the bone tissue around the biomaterial showed all the phases of the bone healing process. In some areas, randomly organized collagen fibers were present, while in other areas, newly formed compact bone was present. In the first bone lamella collagen fibers contacting the Bio-Oss (R) surface were oriented at 243.73 +/- 7.12 degrees (mean +/- SD), while in the rest of the lamella they were oriented at 288.05 +/- 4.86 degrees (mean +/- SD) with a statistically significant difference of 44.32 degrees (p < 0.001). In the same areas the intensity of gray value was 172.56 +/- 18.15 (mean +/- SD) near the biomaterial surface and 158.71 +/- 21.95 (mean +/- SD) in the other part of the lamella with an unstatistically significant difference of 13.79 (P = 0.071). At the bone-biomaterial interface there was also an electron-dense layer similar to cement lines. This layer had a variable morphology being, in some areas, a thin line, and in other areas, a thick irregular band. The analyses showed that Bio-Oss (R) particles do not interfere with the normal osseous healing process after sinus lift procedures and promote new bone formation. In conclusion, this study serves as a better understanding of the morphollogic characteristics of Bio-Oss (R) and its interaction with the surrounding tissues. (c) 2005 Wiley Periodicals, Inc.