Prevalence and Source of Trypanosome Infections in Field-Captured Vector Flies (Glossina pallidipes) in Southeastern Zambia
Prevalence and Source of Trypanosome Infections in Field-Captured Vector Flies (Glossina pallidipes) in Southeastern Zambia
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DOI：
10.1292/jvms.70.923
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发表时间：
2008-09-01
影响因子：
1.2
通讯作者：
Ohashi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Mekata, Hirohisa;Konnai, Satoru;Ohashi, Kazuhiko
采用聚合酶链反应（PCR）对采采蝇（Glossina pallidipes）中锥虫感染的流行情况进行了评估，采蝇采集自赞比亚地方性锥虫病的Chiawa和Chakwenga。在550克中。pallidipes，58只（10.5%）携带锥虫DNA。用间日锥虫通用型、刚果锥虫草原型、刚果锥虫森林型和刚果锥虫森林型对采采蝇进行了感染率调查。刚果红蜱、刚果红蜱、刚果红蜱和刚果红蜱感染率分别为4.2%（23/550）、4.7%（26/550）、1.1%（6/550）和1.6%（9/550）。确定T. congolense和T.对采蝇间日疟原虫感染后，采集其血粉进行PCR扩增，并对扩增产物进行基因序列分析。序列分析表明，人、象、布法罗、山羊、野猪、大捻角羚和牛等7种哺乳动物的细胞色素B基因（cyt B）均存在。进一步检查了这些地区的主要牲畜山羊，以了解其对传播感染的贡献程度。我们研究了锥虫感染的流行率在国内山羊人口在6个定居点在恰瓦单独。在86只山羊中，T阳性4只（4.6%），T阳性5只（5.8%），T阳性4只（4.6%），T阳性4只（4.6%）。vivax universal、间日疟T.刚果大萨凡纳、森林和基利菲。这些结果表明，媒介蝇锥虫感染的宿主-来源提供了有关感染传播的重要信息。本研究结果将有助于进一步阐明锥虫病的流行病学。
The prevalence of trypanosome infections in tsetse flies, Glossina pallidipes, collected front Chiawa and Chakwenga in Zambia with endemic trypanosomosis was assessed by polymerase chain reaction (PCR). Out of the 550 G. pallidipes, 58 (10.5%) flies were found to harbor trypanosome DNA. Infection rates of tsetse with Trypanosoma vivax universal, Trypanosoma congolense savannah, T congolense forest and T. congolense kilifi were 4.2% (23/550), 4.7% (26/550), 1.1% (6/550) and 1.6% (9/550), respectively. To determine the mammalian hosts of T. congolense and T. vivax infections from the tsetse flies, mammalian mitochondrion DNA of blood meal in these flies were analyzed by PCR and subsequent gene sequence analysis of the amplicons. Sequence analysis showed the presence of cytochrome b gene (cyt b) of 7 different mammalian species such as human, elephant, buffalo, goat, warthog, greater kudu and cattle. Goats which were main livestock in these areas were further examined to know the extent of its contribution ill spreading the infection. We examined the prevalence of trypanosome infections in the domestic goat Population in 6 settlements in Chiawa alone. Of the 86 goats sampled, 4 (4.6%), 5 (5.8%), 4 (4.6%) and 4 (4.6%) were positive for T. vivax universal, T. congolense savannah, forest and kilifi, respectively. These findings showed that the host-source of trypanosome infections in vector fly give a vital information about spread of infection. The result of this study will certainly contribute in elucidating more the epidemiology of trypanosomosis.