Antioxidative potential of lactobacilli isolated from the gut of Indian people
Antioxidative potential of lactobacilli isolated from the gut of Indian people
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DOI：
10.1007/s11033-012-1633-9
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发表时间：
2012-08-01
影响因子：
2.8
通讯作者：
Grover, Sunita
中科院分区：
文献类型：
--
作者：
Achuthan, Anju A.;Duary, Raj Kumar;Grover, Sunita
氧化应激是细胞水平发生退行性疾病的主要原因之一，具有严重的健康影响。本研究旨在研究印度肠道益生菌乳酸菌在氧化应激条件下增强宿主细胞抗氧化防御酶系统的能力。对39种乳酸杆菌培养物对活性氧的抗性进行了评估。大多数培养物对0.4 mM H2O2具有中等到强烈的抗性。分离的乳酸杆菌CH4对H2O2的抗性最强，仅降低0.06 log循环。大多数培养物对羟基离子表现出较高的抗性，Lp21的抗性最强，对数计数仅减少0.20倍。几乎所有的培养物都对超氧阴离子有很强的抵抗力。Lp21的超氧化物歧化酶含量最高（0.8971 U）。在39个培养物中，乳杆菌S3的总抗氧化活性最高，为77.85 +/- A 0.13%，其次是Lp55 (56.1 +/- A 1.2%)，对亚麻酸氧化的抑制率为1%。Lp9在0.1 mM（1.997倍）和1.0 mM H2O2（2.058倍）浓度下均上调HT-29细胞超氧化物歧化酶2基因的表达。以谷胱甘肽过氧化物酶-1为例，在0.1 mM H2O2刺激HT-29时，Lp9、Lp91和Lp55的基因表达量显著上调（P < 0.001），分别达到5.451倍、8.706倍和10.083倍。在0.1 mM H2O2条件下，过氧化氢酶基因的表达也显著上调。由此可见，益生菌乳酸菌的抗氧化作用在不同菌种之间存在较大差异，可以作为抗氧化应激诱导疾病的潜在菌株。
Oxidative stress is one of the major causes of degenerative conditions occurring at cellular level with serious health implications. This study was aimed at investigating the antioxidative potentials of probiotic lactobacilli of Indian gut origin and their ability to augment antioxidant defense enzyme systems in the host cells under oxidative stress conditions. A total of 39 Lactobacillus cultures were assessed for their resistance against reactive oxygen species. Most of the cultures were moderately to strongly resistant towards 0.4 mM H2O2. The Lactobacillus isolate CH4 was the most H2O2 resistant culture with only 0.06 log cycle reduction. Majority of the cultures demonstrated high resistance towards hydroxyl ions and Lp21 was the most resistant with log count reduction of 0.20 fold only. Almost all the cultures were also quite resistant to superoxide anions. Lp21 also showed the highest superoxide dismutase content (0.8971 U). Amongst the 39 cultures, Lactobacillus spp. S3 showed the highest total antioxidative activity of 77.85 +/- A 0.13 % followed by Lp55 (56.1 +/- A 1.2 %) in terms of per cent inhibition of linolenic acid oxidation. Lp9 up-regulated the expression of superoxide dismutase 2 gene in HT-29 cells both at 0.1 mM (1.997 folds) and 1.0 mM H2O2 (2.058 folds) concentrations. In case of glutathione peroxidase-1, Lp9, Lp91 and Lp55 showed significant (P < 0.001) up-regulation in the gene expression to the level of 5.451, 8.706 and 10.083 folds, respectively when HT-29 was challenged with 0.1 mM H2O2. The expression of catalase gene was also significantly up-regulated by all the cultures at 0.1 mM H2O2 conditions. It can be concluded that the antioxidative efficacy of the putative probiotic lactobacilli varied considerably between species and strains and the potential strains can be explored as prospective antioxidants to manage oxidative stress induced diseases.