Three-dimensional force microscopy of cells in biopolymer networks
Three-dimensional force microscopy of cells in biopolymer networks
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DOI：
10.1038/nmeth.3685
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发表时间：
2016-02-01
期刊：
影响因子：
48
通讯作者：
Fabry, Ben
中科院分区：
文献类型：
--
作者：
Steinwachs, Julian;Metzner, Claus;Fabry, Ben
我们描述了一种用于定量测量高度非线性三维生物聚合物网络中的细胞生成力的技术，该网络模拟活细胞的生理状况。我们使用基于本构方程的有限元方法，根据测量的细胞周围网络变形计算了MDA-MB-231乳腺癌细胞的力，该本构方程捕获了不同生物聚合物(如胶原凝胶、纤维蛋白凝胶和Matrigel)的复杂机械性能。我们的测量显示，在胶原胶原凝胶中培养的乳腺癌细胞产生几乎恒定的力，而与胶原蛋白浓度和基质硬度无关。此外，时移力测量显示，这些细胞以滑动运动的方式迁移，具有高和低收缩、伸长、迁移速度和持久性的交替阶段。
We describe a technique for the quantitative measurement of cell-generated forces in highly nonlinear three-dimensional biopolymer networks that mimic the physiological situation of living cells. We computed forces of MDA-MB-231 breast carcinoma cells from the measured network deformations around the cells using a finite-element approach based on a constitutive equation that captures the complex mechanical properties of diverse biopolymers such as collagen gels, fibrin gels and Matrigel. Our measurements show that breast carcinoma cells cultured in collagen gels generated nearly constant forces regardless of the collagen concentration and matrix stiffness. Furthermore, time-lapse force measurements showed that these cells migrated in a gliding motion with alternating phases of high and low contractility, elongation, migratory speed and persistence.