DELTA, A TRANSCRIPTION FACTOR THAT BINDS TO DOWNSTREAM ELEMENTS IN SEVERAL POLYMERASE-II PROMOTERS, IS A FUNCTIONALLY VERSATILE ZINC FINGER PROTEIN
DELTA, A TRANSCRIPTION FACTOR THAT BINDS TO DOWNSTREAM ELEMENTS IN SEVERAL POLYMERASE-II PROMOTERS, IS A FUNCTIONALLY VERSATILE ZINC FINGER PROTEIN
复制标题
DOI：
10.1073/pnas.88.21.9799
复制
发表时间：
1991-11-01
影响因子：
11.1
通讯作者：
PERRY, RP
中科院分区：
文献类型：
--
作者：
HARIHARAN, N;KELLEY, DE;PERRY, RP
由RNA聚合酶II转录的几个真核基因的启动子含有位于转录起始点下游的元件。为了深入了解这些元件在形成活性转录复合体中的作用，我们克隆并测定了编码Delta的cDNA，Delta是一种与小鼠核糖体蛋白rpL30和rpL32基因中关键的下游启动子元件结合的蛋白质。我们的结果显示，Delta-蛋白包含四个C-末端的锌指，这是其DNA结合能力所必需的，以及一个非常不寻常的N-末端结构域，包括11个连续的带负电荷的氨基酸和12个连续的组氨酸。德尔塔蛋白的序列被发现与同时克隆的人类转录因子基本相同，该转录因子在免疫球蛋白增强剂和病毒启动子的背景下起到积极和消极的作用。我们对这种蛋白质的结构建模表明，它的性质可以赋予它精致的功能多功能性。
The promoters of several eukaryotic genes transcribed by RNA polymerase II contain elements located downstream of the transcriptional start site. To gain insight into how these elements function in the formation of an active transcription complex, we have cloned and sequenced the cDNA that encodes delta, a protein that binds to critical downstream promoter elements in the mouse ribosomal protein rpL30 and rpL32 genes. Our results revealed that the delta-protein contains four C-terminal zinc fingers, which are essential for its DNA binding capability and a very unusual N-terminal domain that includes stretches of 11 consecutive negatively charged amino acids and 12 consecutive histidines. The sequence of the delta-protein was found to be essentially identical to a concurrently cloned human transcription factor that acts both positively and negatively in the context of immunoglobulin enhancers and a viral promoter. Our structural modeling of this protein indicates properties that could endow it with exquisite functional versatility.