TRIM29 as a novel prostate basal cell marker for diagnosis of prostate cancer
TRIM29 as a novel prostate basal cell marker for diagnosis of prostate cancer
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DOI：
10.1016/j.acthis.2013.12.009
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Hatakeyama, Shigetsugu
中科院分区：
文献类型：
--
作者：
Kanno, Yukiko;Watanabe, Masashi;Hatakeyama, Shigetsugu
三分基序蛋白29（TRIM 29）是TRIM家族蛋白之一，其中一些蛋白具有E3泛素连接酶的功能。在本研究中，我们研究了TRIM 29用于诊断前列腺癌的有用性。使用通过针吸活检和根治性前列腺切除术获得的前列腺组织，包括癌和非癌组织。根据标准程序使用针对TRIM 29的抗体进行免疫组织化学。用抗34 β E12的抗体进行免疫组织化学染色，该抗体识别细胞角蛋白1、5、10和14，作为对照。在所有情况下，正常前列腺腺体的基底细胞用抗TRIM 29抗体染色，而前列腺癌组织用抗TRIM 29抗体没有或几乎没有染色。TRIM29选择性地在正常前列腺的基底细胞中表达，并且用抗TRIM29抗体进行的免疫组织化学染色显示出与前列腺癌及其良性模拟物中的34 β E12相同的表达模式。我们的数据表明，TRIM29可能有助于区分前列腺癌和良性组织。(C)2013年爱思唯尔有限公司。All rights reserved.
Tripartite motif protein 29 (TRIM29) is one of the TRIM family proteins, some of which function as E3 ubiquitin ligases. In this study, we investigated the usefulness of TRIM29 for diagnosis of prostate cancer Prostate tissues including carcinoma and non-carcinoma tissues obtained by needle biopsy and radical prostatectomy were used. Immunohistochemistry was performed according to standard procedures using an antibody against TRIM29. Immunohistochemical staining with an antibody against 34 beta E12, which recognizes cytokeratins 1, 5, 10 and 14, was performed as a control. Basal cells of normal prostatic glands were stained with anti-TRIM29 antibody in all cases, whereas prostate cancer tissues had no or little staining with anti-TRIM29 antibody. TRIM29 is selectively expressed in basal cells of the normal prostate gland, and immunohistochemical staining with anti-TRIM29 antibody showed the same expression pattern as that with 34 beta E12 in prostate cancer and its benign mimics. Our data indicate that TRIM29 may be useful for distinguishing prostate cancers from benign tissues. (C) 2013 Elsevier GmbH. All rights reserved.