Functional role of acetylcholine and the expression of cholinergic receptors and components in osteoblasts
Functional role of acetylcholine and the expression of cholinergic receptors and components in osteoblasts
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DOI：
10.1016/j.febslet.2010.01.001
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发表时间：
2010-02-19
期刊：
影响因子：
3.5
通讯作者：
Yoda, Tetsuya
中科院分区：
文献类型：
--
作者：
Sato, Tsuyoshi;Abe, Takahiro;Yoda, Tetsuya
近年来的研究表明乙酰胆碱（acetylcholine, ACh）在多种组织中起着至关重要的作用，而ACh在骨代谢中的作用尚不清楚。本研究证明乙酰胆碱通过烟碱受体（nAChRs）和毒蕈碱乙酰胆碱受体（mAChRs）诱导成骨细胞增殖并降低碱性磷酸酶（ALP）活性。我们检测了几种nachr和machr的mRNA表达。此外，我们发现在成骨细胞分化过程中，胆碱能成分上调，乙酰胆碱受体亚单位/亚型发生改变。据我们所知，这是首次报道表明成骨细胞表达特异性乙酰胆碱受体和胆碱能成分，乙酰胆碱可能在调节成骨细胞的增殖和分化中发挥作用。皇冠版权(C) 2010由爱思唯尔b.v.代表欧洲生化学会联合会出版。版权所有。
Recent studies have indicated that acetylcholine (ACh) plays a vital role in various tissues, while the role of ACh in bone metabolism remains unclear. Here we demonstrated that ACh induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) and muscarinic acetylcholine receptors (mAChRs) in osteoblasts. We detected mRNA expression of several nAChRs and mAChRs. Furthermore, we showed that cholinergic components were up-regulated and subunits/subtypes of acetylcholine receptors altered during osteoblast differentiation. To our knowledge, this is the first report demonstrating that osteoblasts express specific acetylcholine receptors and cholinergic components and that ACh plays a possible role in regulating the proliferation and differentiation of osteoblasts. Crown Copyright (C) 2010 Published by Elsevier B. V. on behalf of Federation of European Biochemical society. All rights reserved.