Performance metrics for liquid chromatography-tandem mass spectrometry systems in proteomics analyses.
Performance metrics for liquid chromatography-tandem mass spectrometry systems in proteomics analyses.
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DOI：
10.1074/mcp.m900223-mcp200
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发表时间：
2010-02
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影响因子：
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通讯作者：
Stein SE
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文献类型：
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作者：
Rudnick PA;Clauser KR;Kilpatrick LE;Tchekhovskoi DV;Neta P;Blonder N;Billheimer DD;Blackman RK;Bunk DM;Cardasis HL;Ham AJ;Jaffe JD;Kinsinger CR;Mesri M;Neubert TA;Schilling B;Tabb DL;Tegeler TJ;Vega-Montoto L;Variyath AM;Wang M;Wang P;Whiteaker JR;Zimmerman LJ;Carr SA;Fisher SJ;Gibson BW;Paulovich AG;Regnier FE;Rodriguez H;Spiegelman C;Tempst P;Liebler DC;Stein SE
基于LC-MS/MS的蛋白质组学分析中一个主要未满足的需求是一套用于定量评估系统性能和评估技术可变性的工具。在这里，我们描述了46个系统性能指标，用于监测色谱性能、电喷雾源稳定性、MS 1和MS 2信号、MS/MS离子动态采样和肽鉴定。应用于重复LC-MS/MS分析的数据集，这些指标显示出对受控扰动的一致、合理的响应。这些指标通常显示的变化小于10%，因此可以揭示系统组件性能的细微差异。根据共同标准操作程序进行的实验室间研究的数据分析确定了离群数据，并提供了具体原因的线索。此外，由度量反映的实验室间变化指示哪些系统组件在实验室之间变化最大。这些指标的应用使得蛋白质组学和其他LC-MS/MS分析应用能够进行合理的定量质量评估。
A major unmet need in LC-MS/MS-based proteomics analyses is a set of tools for quantitative assessment of system performance and evaluation of technical variability. Here we describe 46 system performance metrics for monitoring chromatographic performance, electrospray source stability, MS1 and MS2 signals, dynamic sampling of ions for MS/MS, and peptide identification. Applied to data sets from replicate LC-MS/MS analyses, these metrics displayed consistent, reasonable responses to controlled perturbations. The metrics typically displayed variations less than 10% and thus can reveal even subtle differences in performance of system components. Analyses of data from interlaboratory studies conducted under a common standard operating procedure identified outlier data and provided clues to specific causes. Moreover, interlaboratory variation reflected by the metrics indicates which system components vary the most between laboratories. Application of these metrics enables rational, quantitative quality assessment for proteomics and other LC-MS/MS analytical applications.