Increased expression of the src proto-oncogene in hairy cell leukemia and a subgroup of B-cell lymphomas.
Increased expression of the src proto-oncogene in hairy cell leukemia and a subgroup of B-cell lymphomas.
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发表时间：
1993-09
期刊：
影响因子：
11.4
通讯作者：
Lynch Sa;Joan S. Brugge;F. Fromowitz;L. Glantz;P. Wang;R. Caruso;Viola Mv
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文献类型：
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作者：
Lynch Sa;Joan S. Brugge;F. Fromowitz;L. Glantz;P. Wang;R. Caruso;Viola Mv
c-src原癌基因编码一个M（r）60，000磷蛋白，pp 60 c-src，具有酪氨酸特异性蛋白激酶活性。我们使用免疫复合物蛋白激酶测定pp 60 c-src来分析B细胞肿瘤的光谱。pp 60 c-src活性在所有五个毛细胞白血病标本和一些大细胞和免疫母细胞淋巴瘤中升高;肿瘤代表B细胞发育的后期阶段。pp 60 c-src活性在对应于B细胞发育的早期和中期阶段（急性和慢性淋巴性白血病、淋巴母细胞性淋巴瘤、小淋巴细胞性淋巴瘤）的肿瘤细胞中较低。pp 60 c-src活性的增强与pp 60 c-src蛋白的高水平相关。然而，c-src的表达增加与c-src基因的扩增或总体结构重排无关。这项初步研究表明pp 60 c-src蛋白和酪氨酸蛋白激酶活性水平升高的肿瘤对应的B细胞个体发育的后期阶段。
The c-src proto-oncogene encodes a M(r) 60,000 phosphoprotein, pp60c-src, with tyrosine-specific protein kinase activity. We have used an immune complex protein kinase assay for pp60c-src to analyze a spectrum of B-cell neoplasms. pp60c-src activity was elevated in all five hairy cell leukemia specimens and in a number of the large cell and immunoblastic lymphomas; neoplasms representing later stages in B-cell development. pp60c-src activity was low in neoplastic cells which correspond to early and intermediate stages in B-cell development (acute and chronic lymphatic leukemia, lymphoblastic lymphoma, small lymphocytic lymphoma). The enhanced pp60c-src activity was associated with high levels of pp60c-src protein. However, increased expression of c-src was not associated with amplification or gross structural rearrangement of the c-src gene. This preliminary study demonstrates elevated levels of pp60c-src protein and tyrosine protein kinase activity in neoplasms corresponding to the later stages of B-cell ontogeny.