Requirement of borate cross-linking of cell wall rhamnogalacturonan II for Arabidopsis growth
Requirement of borate cross-linking of cell wall rhamnogalacturonan II for Arabidopsis growth
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DOI：
10.1126/science.1062319
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发表时间：
2001-10-26
期刊：
影响因子：
56.9
通讯作者：
Darvill, AG
中科院分区：
文献类型：
--
作者：
O'Neill, MA;Eberhard, S;Darvill, AG
能量驱动的植物细胞生长依赖于瓦特结构。两个等位基因L-聚焦缺陷拟南芥突变体（mur1-1和mur1- 2）矮化，其莲座叶不能正常生长。mur1叶细胞瓦特含有正常量的细胞瓦特果胶多糖鼠李糖半乳草酸酯II (RG-II)，但只有一半以硼酸交联二聚体存在。mur1 RG-II结构的改变降低了这种交联的形成速度和稳定性。外源性硼酸盐水溶液修复了该缺陷。矮秆mur1植株RG-II交联降低表明植物生长依赖于果胶多糖组织。
Turgor-driven plant cell growth depends on watt structure. Two allelic L-fucose-deficient Arabidopsis thaliana mutants (mur1-1 and 1-2) are dwarfed and their rosette leaves do not grow normally. mur1 leaf cell watts contain normal amounts of the cell watt pectic polysaccharide rhamnogalacturonan II (RG-II), but only half exists as a borate cross-linked dimer. The altered structure of mur1 RG-II reduces the rate of formation and stability of this cross-link. Exogenous aqueous borate rescues the defect. The reduced cross-linking of RG-II in dwarf mur1 plants indicates that plant growth depends on watt pectic polysaccharide organization.