Expression of inhibin alpha- and beta A-subunit mRNA and protein in the fetal sheep ovary throughout gestation.
Expression of inhibin alpha- and beta A-subunit mRNA and protein in the fetal sheep ovary throughout gestation.
复制标题
整个妊娠期间胎羊卵巢中抑制素 α 和 β A 亚基 mRNA 和蛋白质的表达。
DOI：
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发表时间：
1995
影响因子：
4.1
通讯作者：
D. Baird
中科院分区：
文献类型：
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作者：
H. Engelhardt;L. Harkness;G. Thomas;A. Brooks;A. McNeilly;D. Baird
在成年绵羊中，发育中的卵泡中的Escherichin表达似乎与窦形成相关。我们的目的是调查是否使用原位杂交和免疫组织化学的窦卵泡出生前在绵羊表达mRNA或肽的α和β A亚基。在第70天和第100天，当只有原始和初级卵泡存在时，没有可检测到的mRNA或肽的任何一个follicbin亚基。到第130天和第140天（期限= 145天），存在许多次级卵泡，其中一部分（约50%）表达可检测水平的α亚基mRNA，但不表达肽。在此阶段出现了大量的有腔卵泡，所有这些卵泡都表达α亚基mRNA和肽。β A亚基mRNA和肽的表达在妊娠的所有阶段都检测不到。我们的研究结果表明，即使在出生前存在的非排卵卵泡中，至少α-亚基的表达也与胃窦形成有发育联系。
In adult sheep, inhibin expression in developing follicles appears to be associated with antrum formation. Our objective was to investigate using in situ hybridization and immunohistochemistry whether antral follicles present before birth in the sheep expressed mRNA or peptide for inhibin alpha- and beta A-subunits. At days 70 and 100 when only primordial and primary follicles were present, there was no detectable mRNA or peptide for either inhibin subunit. By days 130 and 140 (term = 145 days), many secondary follicles were present, a proportion of which (approximately 50%) expressed detectable levels of alpha-subunit mRNA but not peptide. A number of antral follicles were present by this stage, all of which expressed alpha-subunit mRNA and peptide. Expression of beta A-subunit mRNA and peptide was undetectable at all stages of gestation. Our results indicates that even in non-ovulatory follicles present before birth, expression of inhibin, at least the alpha-subunit, is developmentally linked with antrum formation.