Elevation of muscle temperature stimulates muscle glucose uptake in vivo and in vitro
Elevation of muscle temperature stimulates muscle glucose uptake in vivo and in vitro
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DOI：
10.1007/s12576-013-0278-3
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发表时间：
2013-11-01
影响因子：
2.3
通讯作者：
Kawanaka, Kentaro
中科院分区：
文献类型：
--
作者：
Koshinaka, Keiichi;Kawamoto, Emi;Kawanaka, Kentaro
本研究的目的是研究肌肉温度升高本身是否可能是增加肌肉葡萄糖摄取的刺激因素。热刺激大鼠后肢增加了体内测量的指长伸肌（EDL）和比目鱼肌的葡萄糖摄取，肌肉温度显著升高。在体内热刺激后立即在离体肌肉中测量葡萄糖摄取时，再次观察到这种热效应。当对孤立的EDL肌肉施加热刺激时，葡萄糖的摄取与肌肉温度的升高成正比。热刺激导致amp活化的蛋白激酶（AMPK）和Akt磷酸化显著扩增，而化合物C、wortmannin或LY294002处理部分阻断了肌肉葡萄糖摄取的热效应。我们提供的证据表明，肌肉温度的升高本身可以直接刺激肌肉葡萄糖的摄取，并且这种热效应是复合C-， wortmannin-和ly294002抑制的。
The purpose of this study was to examine whether elevation of muscle temperature per se might be a stimulatory factor to increase muscle glucose uptake. Heat stimulation to rat hindlimbs increased glucose uptake measured in vivo in the extensor digitorum longus (EDL) and soleus muscles with a significant increase in muscle temperature. This thermal effect was observed again when glucose uptake was measured in vitro in both isolated muscles immediately after the heat stimulation in vivo. When heat stimulation was imposed on isolated EDL muscles, glucose uptake was facilitated in proportion to the increase in muscle temperature. The heat stimulation led to a significant amplification in the phosphorylation of AMP-activated protein kinase (AMPK) and Akt, and treatment with compound C, wortmannin, or LY294002 partially blocked the thermal effect on muscle glucose uptake. We provide evidence that elevation of muscle temperature per se can directly stimulate muscle glucose uptake and that this thermal effect is compound C-, wortmannin-, and LY294002-inhibitable.