Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models.
Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models.
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DOI：
10.5555/uri:pii:0016508584902026
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发表时间：
1984
期刊：
影响因子：
29.4
通讯作者：
J. E. Krawisz;P. Sharon;W. Stenson
中科院分区：
文献类型：
--
作者：
J. E. Krawisz;P. Sharon;W. Stenson
设计了一种基于髓过氧化物酶活性评估定量急性肠道炎症的测定法。髓过氧化物酶是一种在中性粒细胞中发现的酶，在单核细胞和巨噬细胞中也有少量存在。用十六烷基三甲基溴化铵溶解髓过氧化物酶，并用联茴香胺-H2 O2测定法测定髓过氧化物酶活性。在中性粒细胞悬液中，髓过氧化物酶活性与细胞数量直接相关，最低可达500个细胞。在两种动物炎症模型中测定了髓过氧化物酶活性：乙酸诱导的大鼠结肠炎和艰难梭菌肠毒素诱导的仓鼠肠炎。在这两种模型中，从发炎组织中溶解的髓过氧化物酶的活性与组织切片中观察到的中性粒细胞数量成正比。通过对0.18 mm长、5 μ m厚的组织切片中的中性粒细胞数量进行计数，对中性粒细胞蓄积进行组织学评价。在这两种动物模型中，髓过氧化物酶活性与400和4000个细胞/mm的中性粒细胞数量呈线性相关。慢性炎症结肠，其中中性粒细胞和组织细胞都存在，髓过氧化物酶活性与中性粒细胞含量直接相关。组织细胞对髓过氧化物酶活性无显著影响。肠内髓过氧化物酶活性的测定是一种简单的生化测定，可用于定量炎症。
An assay was devised to quantitate acute intestinal inflammation based on the assessment of myeloperoxidase activity. Myeloperoxidase is an enzyme found in neutrophils and, in much smaller quantities, in monocytes and macrophages. Myeloperoxidase was solubilized with hexadecyltrimethylammonium bromide and myeloperoxidase activity was measured with a dianisidine-H2O2 assay. In neutrophil suspensions, myeloperoxidase activity was directly related to cell number down to as few as 500 cells. Myeloperoxidase activity was assayed in two animal models of inflammation: acetic acid-induced colitis in rats and Clostridium difficile enterotoxin-induced enteritis in hamsters. In both models, the activity of myeloperoxidase solubilized from the inflamed tissue was directly proportional to the number of neutrophils seen in histologic sections. Histologic evaluation of neutrophil accumulation was performed by counting the number of neutrophils in a histologic section 0.18 mm long and 5 micron thick. In both animal models, myeloperoxidase activity was linearly related to neutrophil number from 400 and 4000 cells/mm. Myeloperoxidase activity from chronically inflamed colon, in which both neutrophils and histiocytes were present, was directly related to neutrophil content. Histiocytes did not contribute significantly to myeloperoxidase activity. The determination of myeloperoxidase activity in the intestine is a simple biochemical assay that can be used to quantitate inflammation.