Drosophila melanogaster Selection for Survival of Bacillus cereus Infection: Life History Trait Indirect Responses.
Drosophila melanogaster Selection for Survival of Bacillus cereus Infection: Life History Trait Indirect Responses.
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DOI：
10.1155/2012/935970
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Harshman, Lawrence G
中科院分区：
文献类型：
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作者：
Ma, Junjie;Benson, Andrew K;Harshman, Lawrence G
为了研究昆虫模型中进化的抗性/耐受性，我们使用黑腹果蝇和机会性病原体蜡样芽孢杆菌作为选择剂进行了实验进化研究。经过 18-24 代选择后，选定的品系进化出 50% 死亡率所需的孢子浓度增加 3.0 至 3.3 个对数。在没有任何处理的情况下，选定的品系进化出产蛋量增加并延迟发育时间。后一种反应可以被解释为进化的成本。或者，发育迟缓可能是选择的目标，导致成年脂肪体功能增加，包括抗菌肽的产生，以及卵母细胞和卵子的蛋黄产生。当用高压灭菌的孢子处理时，选定品系和对照品系之间的产卵差异被消除，并且这种反应与诱导免疫反应的成本假设一致。在某些情况下，用高压灭菌的孢子进行治疗还缩短了寿命，并在选定的和伤口对照系中引起了早期死亡率，这两者都与与诱导免疫反应相关的成本的假设一致。一般来说，产蛋量测定得出了关键结果，包括高压灭菌孢子对产蛋量的负面影响。
To study evolved resistance/tolerance in an insect model, we carried out an experimental evolution study using D. melanogaster and the opportunistic pathogen B. cereus as the agent of selection. The selected lines evolved a 3.0- to 3.3-log increase in the concentration of spores required for 50% mortality after 18-24 generations of selection. In the absence of any treatment, selected lines evolved an increase in egg production and delayed development time. The latter response could be interpreted as a cost of evolution. Alternatively, delayed development might have been a target of selection resulting in increased adult fat body function including production of antimicrobial peptides, and, incidentally, yolk production for oocytes and eggs. When treated with autoclaved spores, the egg production difference between selected and control lines was abolished, and this response was consistent with the hypothesis of a cost of an induced immune response. Treatment with autoclaved spores also reduced life span in some cases and elicited early-age mortality in the selected and wound-control lines both of which were consistent with the hypothesis of a cost associated with induction of immune responses. In general, assays on egg production yielded key outcomes including the negative effect of autoclaved spores on egg production.