A Synthetic Oxygen Sensor for Plants Based on Animal Hypoxia Signaling
A Synthetic Oxygen Sensor for Plants Based on Animal Hypoxia Signaling
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DOI：
10.1104/pp.18.01003
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发表时间：
2019-03-01
期刊：
影响因子：
7.4
通讯作者：
Licausi, Francesco
中科院分区：
文献类型：
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作者：
Iacopino, Sergio;Jurinovich, Sandro;Licausi, Francesco
由于氧参与了许多重要的代谢反应，所有活着的生物体都发展了分子系统，允许根据氧气的可获得性进行适应性的生理和代谢转变。在哺乳动物中，低氧反应基因的表达受异源二聚体低氧诱导因子的控制。这种转录调节因子的活性主要与其a亚单位保守的脯氨酸残基的氧依赖羟化有关，这是由Pro-羟基酶执行的，随后通过E3连接酶von Hippel-Lindau肿瘤抑制因子而泛素化，该连接酶将缺氧诱导因子-a靶向蛋白酶体。通过利用这种哺乳动物氧传感器的生物工程版本，我们设计并优化了一种合成设备，它以依赖于氧的方式在植物中驱动基因表达。对拟南芥叶肉原生质体的瞬时分析表明，酵母Gal4/上游激活序列系统和哺乳动物氧感受器机械的结合可以有效地设计出一个模块化的、氧诱导的转录调节因子。当其成分在拟南芥幼苗中稳定表达时，这种合成装置也被证明在整个植物中选择性地受氧控制。我们设想利用我们的遗传编码控制器来产生能够根据氧气供应选择性地切换基因表达的植物，从而为合成生物学在氧气供应可变的环境中辅助农业实践的潜力提供概念证明。
Due to the involvement of oxygen in many essential metabolic reactions, all living organisms have developed molecular systems that allow adaptive physiological and metabolic transitions depending on oxygen availability. In mammals, the expression of hypoxia-response genes is controlled by the heterodimeric Hypoxia-Inducible Factor. The activity of this transcriptional regulator is linked mainly to the oxygen-dependent hydroxylation of conserved proline residues in its a-subunit, carried out by prolyl-hydroxylases, and subsequent ubiquitination via the E3 ligase von Hippel-Lindau tumor suppressor, which targets Hypoxia-Inducible Factor-a to the proteasome. By exploiting bioengineered versions of this mammalian oxygen sensor, we designed and optimized a synthetic device that drives gene expression in an oxygen-dependent fashion in plants. Transient assays in Arabidopsis (Arabidopsis thaliana) mesophyll protoplasts indicated that a combination of the yeast Gal4/upstream activating sequence system and the mammalian oxygen sensor machinery can be used effectively to engineer a modular, oxygen-inducible transcriptional regulator. This synthetic device also was shown to be selectively controlled by oxygen in whole plants when its components were expressed stably in Arabidopsis seedlings. We envision the exploitation of our genetically encoded controllers to generate plants able to switch gene expression selectively depending on oxygen availability, thereby providing a proof of concept for the potential of synthetic biology to assist agricultural practices in environments with variable oxygen provision.