Surgisis patch tracheoplasty in a rodent model for tracheal stenosis.
Surgisis patch tracheoplasty in a rodent model for tracheal stenosis.
复制标题
在啮齿动物模型中进行气管狭窄手术补片气管成形术。
DOI：
10.1016/s0022-4804(03)00107-0
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
J. Dunn
中科院分区：
文献类型：
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作者：
Daniel A. De Ugarte;Devin P. Puapong;J. Roostaeian;Nicole Gillis;E. Fonkalsrud;J. Atkinson;J. Dunn
背景 气管狭窄是一个具有挑战性的外科问题，可能需要使用自体移植物或人工支架进行重建。在这项研究中，我们评估了 Surgisis（一种市售的生物相容性脱细胞基质）在修复临界尺寸气管缺损中的功效。方法 在成年大鼠气管环 4 至 6 中制造全层缺损（2 mm × 6 mm）。用间断的 8-0 聚丙烯缝线将一块 8 层 Surgisis（Cook；布卢明顿，印第安纳州）缝合到缺损的边缘。在对照动物中，缺陷被初步闭合。 4周时收获气管并准备使用常规技术进行组织学评估。使用成像软件计算横截面积和周长。结果 未进行补片修复的气管缺陷（n = 3）导致气管狭窄并立即死亡。接受气管缺陷外科补片修复术的动物 (n = 10) 对该手术的耐受性良好，并且没有可听到的喘鸣声或呼吸窘迫的证据。十只动物中有八只存活了 4 周。气管内腔是通畅的，没有证据表明手术有挛缩或退化。组织学上，Surgisis 的新生血管形成伴有中度炎症。手术贴片的表面覆盖有纤毛上皮细胞的衬里。结论 在啮齿动物模型中，Surgisis 似乎是补片修复部分环形气管缺损的有效方法。手术似乎是促进新血管形成和组织再生的安全且有前途的方法。 Surgisis 的长期使用值得进一步研究。
Background Tracheal stenosis is a challenging surgical problem that can require reconstruction using autologous grafts or artificial stents. In this study, we evaluate the efficacy of Surgisis, a commercially available, biocompatible, acellular matrix, in the repair of a critical-size tracheal defect. Methods A full-thickness defect (2 mm × 6 mm) was created in tracheal rings 4 through 6 in adult rats. A piece of 8-ply Surgisis (Cook; Bloomington, IN) was sutured to the edges of the defect with interrupted 8-0 polypropylene sutures. In control animals, the defect was closed primarily. The trachea was harvested at 4 weeks and prepared for histologic evaluation using conventional techniques. Cross-sectional area and perimeters were calculated using imaging software. Results Tracheal defects without patch repair (n = 3) resulted in tracheal stenosis and immediate death. Animals that underwent Surgisis patch repair of tracheal defects (n = 10) tolerated the procedure well and had no audible stridor or evidence of respiratory distress. Eight of ten animals survived 4 weeks. The tracheal lumen was patent with no evidence of contracture or degradation of the Surgisis. Histologically, neovascularization of the Surgisis was noted with moderate inflammation. The surface of the Surgisis patch was covered ith a lining of ciliated epithelial cells. Conclusion In the rodent model, Surgisis appears to be an efficacious method for the patch repair of partial circumferential tracheal defects. Surgisis appears to be a safe and promising means of facilitating neovascularization and tissue regeneration. The long-term use of Surgisis warrants further investigation.