cAMP regulates soluble guanylate cyclase beta 1-subunit gene expression in RFL-6 rat fetal lung fibroblasts.
cAMP regulates soluble guanylate cyclase beta 1-subunit gene expression in RFL-6 rat fetal lung fibroblasts.
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cAMP 调节 RFL-6 大鼠胎肺成纤维细胞中可溶性鸟苷酸环化酶 β1 亚基基因表达。
DOI：
10.1152/ajplung.1993.265.5.l456
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Bloch,KD
中科院分区：
文献类型：
--
作者：
Shimouchi,A;Janssens,SP;Bloch,DB;Zapol,WM;Bloch,KD
内皮源性舒张因子（EDRF）/一氧化氮（NO）激活可溶性鸟苷酸环化酶，从而刺激鸟苷3 '，5'-环一磷酸（cGMP）的合成。为了研究这种重要的EDRF/NO受体的调节，我们研究了可溶性鸟苷酸环化酶基因在大鼠胎肺成纤维细胞系（RFL-6）中的表达。3-异丁基-1-甲基黄嘌呤、毛喉素和二丁酰腺苷3 '，5'-环一磷酸（cAMP）（增加细胞内cAMP的药物）降低了RFL-6细胞中编码可溶性鸟苷酸环化酶β 1亚基的mRNA的浓度。为了研究β 1亚基mRNA浓度的降低是否反映在产生cGMP的能力降低中，将毛喉素处理的RFL-6细胞暴露于NO供体化合物硝普钠。暴露于毛喉素可逆地降低RFL-6细胞的能力，增加cGMP响应NO。这些观察结果表明，cAMP可以调节细胞反应EDRF/NO通过减少可溶性鸟苷酸环化酶的亚基之一的表达。
Endothelium-derived relaxing factor (EDRF)/nitric oxide (NO) activates soluble guanylate cyclase, thereby stimulating the synthesis of guanosine 3',5'-cyclic monophosphate (cGMP). To investigate the regulation of this important EDRF/NO receptor, we studied soluble guanylate cyclase gene expression in a rat fetal lung fibroblast cell line (RFL-6). 3-Isobutyl-1-methylxanthine, forskolin, and dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP), agents which increase intracellular cAMP, decreased the concentration of mRNA encoding the beta 1-subunit of soluble guanylate cyclase in RFL-6 cells. To investigate whether a decrease in beta 1-subunit mRNA concentration was reflected in diminished capacity to produce cGMP, forskolin-treated RFL-6 cells were exposed to the NO-donor compound sodium nitroprusside. Exposure to forskolin reversibly reduced the ability of RFL-6 cells to increase cGMP in response to NO. These observations suggest that cAMP can modulate the cellular response to EDRF/NO by decreasing the expression of one of the subunits of soluble guanylate cyclase.