Influence of saline infusion on blood-tissue albumin transport.
Influence of saline infusion on blood-tissue albumin transport.
复制标题
盐水输注对血液组织白蛋白转运的影响。
DOI：
10.1152/ajpheart.1989.257.2.h525
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Sibley,L
中科院分区：
文献类型：
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作者：
Renkin,EM;Rew,K;Wong,M;O'Loughlin,D;Sibley,L
以恒定速率向麻醉大鼠输注乳酸林格液（LR）30或60 min;递送的体积负荷范围为0.03 - 0.08 ml/g体重。对照组仅给予0.002 ml.g-1.h-1的生理盐水持续输注。输注结束时，仅7-14%的LR保留在血浆中; 76-88%进入间质室，7-17%排泄。通过两种方法同时研究了随外渗液从循环中丢失的血浆蛋白量：1）整个动物的物质平衡和2）单个组织中131 I标记的白蛋白摄取（VA）和水含量（VW）的变化。0.03-0.06 ml液体/g体重（75-160%初始血浆体积）的外渗未显著增加整个大鼠的血浆蛋白外渗。几乎所有LR输注大鼠的取样组织都比对照组具有更高的VW。组织VA倾向于随VW增加，但回归斜率（Δ VA/Δ VW）（毛细血管滤液相对于血浆的示踪白蛋白浓度的测量值）较低;皮肤，0.006;爪，0.018;骨骼肌，0.007;心脏，0.057;空肠，0.095;回肠，0.045;盲肠，0.026;结肠，0.027。这些比值与观察到的非常小的总血浆蛋白损失一致，并证明了高溶剂阻力反射系数（σ约等于1 -Δ VA/Δ VW）：骨骼肌、皮肤和爪的毛细血管中大于0.98，心脏和肠中为0.91-0.97。
Anesthetized rats were infused with lactated Ringer solution (LR) at constant rate for 30 or 60 min; delivered volume loads ranged from 0.03 to 0.08 ml/g body wt. Controls were given only a sustaining infusion of saline at 0.002 ml.g-1.h-1. Only 7-14% of the LR remained in the plasma at the end of the infusion; 76-88% entered the interstitial compartment, and 7-17% was excreted. The amount of plasma protein lost from the circulation with the extravasated fluid was studied simultaneously by two methods: 1) material balance in the whole animal and 2) changes in 131I-labeled albumin uptake (VA) and water content (VW) in individual tissues. The extravasation of 0.03-0.06 ml fluid/g body wt (75-160% initial plasma volume) did not significantly increase plasma protein extravasation in the whole rat. Nearly all of the sampled tissues of LR-infused rats had higher VW than controls. Tissue VA tended to increase with VW, but the regression slopes (delta VA/delta VW), a measure of the tracer albumin concentration of capillary filtrate relative to plasma, were low; skin, 0.006; paw, 0.018; skeletal muscles, 0.007; heart, 0.057; jejunum, 0.095; ileum, 0.045; cecum, 0.026; and colon, 0.027. These ratios are consistent with the very small loss of total plasma protein observed and attest to high solvent-drag reflection coefficients (sigma approximately equal to 1 - delta VA/delta VW): greater than 0.98 in capillaries of skeletal muscles, skin, and paw and 0.91-0.97 in heart and intestine.