NMR structural analysis of the G.G mismatch DNA complexed with naphthyridine-dimer.
NMR structural analysis of the G.G mismatch DNA complexed with naphthyridine-dimer.
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与萘啶二聚体复合的 G.G 错配 DNA 的 NMR 结构分析。
DOI：
10.1093/nass/49.1.213
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
C. Kojima
中科院分区：
文献类型：
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作者：
M. Nomura;S. Hagihara;Yuki Goto;K. Nakatani;C. Kojima
萘嘧啶二聚体（ND）特异性识别G.G错配DNA（图1）。然而，其详细的识别机制尚不清楚。本文用核磁共振技术研究了DNA低聚物d(CTAACGGAATG)/d（CATTCGGTTAA）与ND的络合。在核磁共振浓度（2.5 mM）下，DNA与ND的化学计量为1:2。利用配合物的1H-1H和1H-13C二维光谱完全确定了质子共振，包括H5’和H5’。在CGG/CGG区，4个萘啶环与4个G残基形成氢键。这些结果表明ND能够特异性识别CGG/CGG序列。
Naphthyridine-dimer (ND) specifically recognizes G.G mismatch DNA (Figure 1). However, its detailed recognition mechanism is not clear. Here a DNA oligomer d(CTAACGGAATG)/d(CATTCGGTTAA) complexed with ND was studied by NMR. The stoichiometry of DNA to ND was determined to be 1:2 at NMR concentration (2.5 mM). Proton resonances were completely assigned including H5' and H5'' using 1H-1H and 1H-13C 2D spectra of the complex. These spectra showed that four naphthyridine rings are staked in the helix and form hydrogen bonds with the four G residues in CGG/CGG region. These results indicate ND can specifically recognize the CGG/CGG sequence.