Melatonin alleviates heat stress-induced oxidative stress and apoptosis in human spermatozoa.
Melatonin alleviates heat stress-induced oxidative stress and apoptosis in human spermatozoa.
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DOI：
10.1016/j.freeradbiomed.2021.01.014
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发表时间：
2021-01
影响因子：
7.4
通讯作者：
F. Zhao;S. Whiting;S. Lambourne;R. Aitken;Yingshuo Sun
中科院分区：
文献类型：
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作者：
F. Zhao;S. Whiting;S. Lambourne;R. Aitken;Yingshuo Sun
氧化应激会产生大量活性氧 (ROS)，并通过破坏精子 DNA 和损害人类精子的细胞内稳态来影响精子质量。在辅助生殖技术（ART）中，防止精子受到ROS攻击具有重要意义。松果体激素褪黑激素具有天然的抗氧化能力，可以清除ROS。然而，据我们所知，目前还没有研究调查褪黑激素是否可以保护人类精子免受热诱导的氧化损伤。在此，我们通过热处理诱导人类精子氧化应激，并确定褪黑激素可以保护人类精子免受热诱导的氧化应激。我们首先证实热应激诱导的氧化应激通过降低精子活力和活力来损害人类精子。此外，褪黑激素对人类精子的预处理能够通过抑制精子线粒体ROS的产生、增加线粒体膜电位、减少脂质过氧化产物4-HNE的形成、减少精子DNA损伤和凋亡来减轻这种损伤。总的来说，这些发现表明褪黑激素可作为治疗热诱导氧化应激引起的男性不育症的潜在治疗选择。
Oxidative stress generates a large amount of reactive oxygen species (ROS) and affects sperm quality via damaging sperm DNA and compromising the intracellular homeostasis in human spermatozoa. In assisted reproductive technology (ART), it is substantial to prevent spermatozoa from ROS attack. The pineal hormone melatonin has the natural antioxidant capacity and can scavenge ROS. To the best of our knowledge, however, there are presently no studies investigating if melatonin can protect human spermatozoa from heat-induced oxidative damage. Herein, we induced oxidative stress in human spermatozoa with heat treatment, and determined that melatonin could protect human spermatozoa from heat-induced oxidative stress. We first confirmed that heat stress-induced oxidative stress damaged human spermatozoa by decreasing sperm motility and viability. Furthermore, the pretreatment of human spermatozoa by melatonin was able to alleviate such damage by suppressing sperm mitochondrial ROS generation, increasing mitochondrial membrane potential, reducing the formation of the lipid peroxidation product, 4-HNE, and reducing sperm DNA damage and apoptosis. Collectively, these findings suggest that melatonin is useful as a potential treatment option for male infertility caused by heat-induced oxidative stress.