Protective efficacy against Chlamydophila psittaci by oral immunization based on transgenic rice expressing MOMP in mice.
Protective efficacy against Chlamydophila psittaci by oral immunization based on transgenic rice expressing MOMP in mice.
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DOI：
10.1016/j.vaccine.2012.11.039
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发表时间：
2013-01
期刊：
影响因子：
5.5
通讯作者：
Xiu-Xiang Zhang;Hui Yu;Xiao-hu Wang;Xiu Li;Yan-Ping Zhu;Hao-Xin Li;Sheng-jun Luo;Zi-Guo Yuan
中科院分区：
文献类型：
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作者：
Xiu-Xiang Zhang;Hui Yu;Xiao-hu Wang;Xiu Li;Yan-Ping Zhu;Hao-Xin Li;Sheng-jun Luo;Zi-Guo Yuan
禽衣原体病是由鹦鹉热嗜衣原体（Chlamecophila psittaci）（Cp. psittaci）和Cp.鹦鹉热是一种很好的疫苗候选者。本研究利用农杆菌介导的方法，在水稻愈伤组织中表达MOMP基因。经Western-blot和ELISA检测，转基因水稻种子中蛋白质的表达量稳定。将表达MOMP蛋白的转基因水稻种子通过口服途径给予BALB/c小鼠，其产生MOMP特异性血清IgG和粪便伊加抗体和脾细胞MOMP特异性增殖反应以及显著水平的IFN-γ、IL-2、IL-4、IL-5和TGF-β产生。免疫MOMP转基因种子诱导部分保护（50%）对致命的挑战与高毒力Cp。鹦鹉热6 BC株。与非MOMP免疫的动物相比，攻击后的肺功能受影响较小。结果表明，转基因水稻种子作为口服疫苗的可行性，以产生保护性免疫，减少对强毒Cp小鼠的肺部病变。鹦鹉热6 BC株。这一发现对进一步开发禽衣原体病口服疫苗具有重要意义。
Avian chlamydiosis is caused by Chlamydophila psittaci (Cp. psittaci) and major outer membrane protein (MOMP) of Cp. psittaci is an excellent vaccine candidate. In this study, the MOMP gene was expressed in rice callus by the Agrobacterium tumefaciens vector. The production of protein in transgenic rice seeds was confirmed and quantified by Western-blot and ELISA, the results demonstrating that the antigen was expressed stably. The transgenic rice seeds expressing the MOMP protein were administered by the oral route to BALB/c mice, which developed MOMP-specific serum IgG and fecal IgA antibodies and a splenocyte MOMP-specific proliferative response and significant levels of IFN-γ, IL-2, IL-4, IL-5 and TGF-β production. Immunization with MOMP transgenic seeds induced partial protection (50%) against a lethal challenge with the highly virulent Cp. psittaci 6BC strain. Lung function after challenge was less affected compared non-MOMP immunized animals. The results demonstrate the feasibility of using transgenic rice seeds as an oral vaccine to generate protective immunity and reduce the lung lesions in mice against virulent Cp. psittaci 6BC strain. This finding has implications for further development of an oral vaccine against avian chlamydiosis.