Immunization with the recombinant antigen Ss-IR induces protective immunity to infection with Strongyloides stercoralis in mice
Immunization with the recombinant antigen Ss-IR induces protective immunity to infection with Strongyloides stercoralis in mice
复制标题
DOI：
10.1016/j.vaccine.2011.08.030
复制
发表时间：
2011-10-19
期刊：
影响因子：
5.5
通讯作者：
Nutman, Thomas B.
中科院分区：
文献类型：
--
作者：
Abraham, David;Hess, Jessica A.;Nutman, Thomas B.
人类肠道感染粪类圆线虫仍然是世界范围内的一个重大问题，疫苗将是预防和控制这种感染的有用工具。本研究的目的是测试单一抗原在抗沙门氏菌疫苗中的效力。粪蝇幼虫。在这些研究中使用明矾作为佐剂，并且选择用于分析的抗原被保护性人IgG识别（Ss-TMY-1、Ss-EAT-6和Ss-LEC-5）或已知在人体中具有高度免疫原性（Ss-NIE-1和Ss-IR）。只有用Ss-IR抗原免疫的小鼠在攻击感染中表现出幼虫寄生虫存活率显著降低约80%。从对S.用Ss-IR免疫后的粪蝇幼虫，用于在幼虫中定位抗原。共聚焦显微镜显示，免疫小鼠的IgG与Ss-IR结合的寄生虫的表面和观察，通过电子显微镜表明，IgG结合的颗粒在腺食管。从用Ss-IR免疫的小鼠收集的血清被动地将免疫转移至幼稚小鼠。这些研究表明，Ss-IR与明矾组合，通过抗体依赖性机制诱导高水平的保护性免疫，因此可能适合于进一步开发作为抗人类类圆线虫病的疫苗。(C)2011爱思唯尔有限公司保留所有权利。
Human intestinal infections with the nematode Strongyloides stercoralis remain a significant problem worldwide and a vaccine would be a useful addition to the tools available to prevent and control this infection. The goal of this study was to test single antigens for their efficacy in a vaccine against S. stercoralis larvae in mice. Alum was used as the adjuvant in these studies and antigens selected for analysis were either recognized by protective human IgG (Ss-TMY-1, Ss-EAT-6, and Ss-LEC-5) or were known to be highly immunogenic in humans (Ss-NIE-1 and Ss-IR). Only mice immunized with the Ss-IR antigen demonstrated a significant decrease of approximately 80% in the survival of larval parasites in the challenge infection. Antibodies, recovered from mice with protective immunity to S. stercoralis after immunization with Ss-IR, were used to locate the antigen in the larvae. Confocal microscopy revealed that IgG from mice immunized with Ss-IR bound to the surface of the parasites and observations by electron microscopy indicated that IgG bound to granules in the glandular esophagus. Serum collected from mice immunized with Ss-IR passively transferred immunity to naive mice. These studies demonstrate that Ss-IR, in combination with alum, induces high levels of protective immunity through an antibody dependent mechanism and may therefore be suitable for further development as a vaccine against human strongyloidiasis. (C) 2011 Elsevier Ltd. All rights reserved.