Characterization of polyamine synthesis pathway in Bacillus subtilis 168
Characterization of polyamine synthesis pathway in Bacillus subtilis 168
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DOI：
10.1046/j.1365-2958.1998.00979.x
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发表时间：
1998-08
影响因子：
3.6
通讯作者：
A. Sękowska;P. Bertin;A. Danchin
中科院分区：
文献类型：
--
作者：
A. Sękowska;P. Bertin;A. Danchin
无处不在的多胺在生命的三个领域中发挥着各种各样的作用。然而，人们对这些化合物在革兰氏阳性细菌中的生物合成知之甚少。我们表明，枯草芽孢杆菌有一个单一的途径到多胺，从精氨酸开始，以胍为中间体。我们首先确定了精氨酸脱羧酶的结构基因speA（原cad），然后描述了speE speB操纵子，指导亚精胺合酶和agmatinase的合成。这个操纵子被转录成两个信使rna，一个主要用于speE基因，一个次要用于speE和speB基因。通过引物延伸分析，确定了speE基因上游的操纵子启动子。该操纵子的转录表明agmatinase合成水平非常低，因此可以严格控制腐胺的合成，从而控制所有多胺的合成。这与细胞中检测到的多胺水平一致。
The ubiquitous polyamines fulfil a variety of functions in all three kingdoms of life. However, little is known about the biosynthesis of these compounds in Gram‐positive bacteria. We show that, in Bacillus subtilisthere is a single pathway to polyamines, starting from arginine, with agmatine as an intermediate. We first identified the structural gene of arginine decarboxylase, speA (formerly cad ), and then described the speE speB operon, directing synthesis of spermidine synthase and agmatinase. This operon is transcribed into two messenger RNAs, a major one for the speE gene and a minor one for both speE and speB. The promoter of the operon was identified upstream from the speE gene by primer extension analysis. Transcription of this operon indicated that the level of agmatinase synthesis is very low, thus allowing a stringent control on the synthesis of putrescine and, therefore, of all polyamines. This is consistent with the level of polyamines measured in the cell.