Vocal fold epithelial response to luminal osmotic perturbation
Vocal fold epithelial response to luminal osmotic perturbation
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DOI：
10.1044/1092-4388(2007/063
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发表时间：
2007-08-01
影响因子：
2.6
通讯作者：
Fisher, Kimberly V.
中科院分区：
文献类型：
--
作者：
Sivasankar, Mahalakshmi;Fisher, Kimberly V.
目的：干燥空气挑战增加了近端气道腔表面内衬液体的渗透压。表面流体的稳态被认为是发声和喉防御所必需的。因此，作者假设活声带上皮会产生水通量，以减少对管腔的渗透挑战（150 mOsm甘露醇）。双向transepithelial水通量测定声带暴露于生理上现实的管腔渗透perturbations in vitro.Method：36本地绵羊声带暴露于管腔高渗或等渗扰动。声带的活力和水通量的管腔和进入粘膜进行了测量，在prechallenge基线和30 min后challense.Results：声带电生理活力保持渗透扰动的持续时间。腔渗透暴露增加了60%的声带腔定向经上皮水通量。这种增加是电沉默，持续时间短，不会否定的渗透gradient.Conclusion：绵羊声带上皮细胞检测渗透扰动的管腔表面在体外。这种检测和响应表面组成变化的能力可能是重要的，在呼吸和发声的渗透扰动过程中的声带表面流体的稳态调节。
Purpose: Dry-air challenges increase the osmolarity of fluid lining the luminal surface of the proximal airway. The homeostasis of surface fluid is thought to be essential for voice production and laryngeal defense. Therefore, the authors hypothesized that viable vocal fold epithelium would generate a water flux to reduce an osmotic challenge (150 mOsm mannitol) on the lumen. Bidirectional transepithelial water fluxes were measured in vocal folds exposed to physiologically realistic luminal osmotic perturbations in vitro.Method: Thirty-six native ovine vocal folds were exposed to either luminal hyperosmotic or isosmotic perturbations. Vocal fold viability and water fluxes toward the lumen and into the mucosa were measured at prechallenge baseline and for 30 min after challenge.Results: Vocal fold electrophysiological viability was maintained for the duration of osmotic perturbation. Luminal osmotic exposure increased luminally directed transepithelial water fluxes in 60% of vocal folds. This increase was electrically silent, of short duration, and would not negate the osmotic gradient.Conclusion: Ovine vocal fold epithelia detect osmotic perturbations to the luminal surface in vitro. This ability to detect and respond to changes in surface composition may be important in homeostatic regulation of vocal fold surface fluid during osmotic perturbations in respiration and phonation.