Establishment of lateral organ asymmetries in the invertebrate chordate, Ciona intestinalis.
Establishment of lateral organ asymmetries in the invertebrate chordate, Ciona intestinalis.
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DOI：
10.1186/s13227-017-0075-9
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发表时间：
2017
期刊：
影响因子：
4.1
通讯作者：
Davidson B
中科院分区：
文献类型：
--
作者：
Palmquist K;Davidson B
脊索动物的进化出现和多样化似乎涉及器官形态发生沿着左/右轴的巨大变化。然而，建立横向不对称的祖先脊索机制仍然不明确。此外，最初建立的横向不对称性和随后的器官形态发生的不对称性之间的联系是很差的特点。为了探索脊索动物进化过程中的不对称器官形态发生，我们已经开始表征左/右图案的心脏和内胚层器官的无脊椎动物脊索动物，玻璃海鞘。在这里，我们表明，玻璃海鞘有一个横向不对称，右侧的心脏。我们的数据表明，心脏的横向不对称性需要H+/K+离子流，但不依赖于Nodal信号。我们的药理学抑制剂的研究表明，离子通量是所需的表皮纤毛和神经元旋转的极化，并建议，离子通量的功能协同绒毛膜接触，以驱动心脏偏侧。实时成像分析显示，幼虫心脏祖细胞进行横向移动，而不显示任何迁移行为。此外，我们发现，这种被动移位对应于内胚层中的横向不对称性的出现，这也是离子通量依赖的。我们的数据表明，离子流促进横向不对称的形态发生的幼虫内胚层雏形导致一个被动的，节点独立的相关心脏祖细胞的位置的转变。这些发现有助于完善关于祖先脊索动物左/右模式机制的假设，以及它们如何在无脊椎动物和脊椎动物脊索动物谱系中分化。本文的在线版本（doi：10.1186/s13227-017-0075-9）包含补充材料，可供授权用户使用。
The evolutionary emergence and diversification of the chordates appear to involve dramatic changes in organ morphogenesis along the left/right axis. However, the ancestral chordate mechanism for establishing lateral asymmetry remains ambiguous. Additionally, links between the initial establishment of lateral asymmetry and subsequent asymmetries in organ morphogenesis are poorly characterized. To explore asymmetric organ morphogenesis during chordate evolution, we have begun to characterize left/right patterning of the heart and endodermal organs in an invertebrate chordate, Ciona intestinalis. Here, we show that Ciona has a laterally asymmetric, right-sided heart. Our data indicate that cardiac lateral asymmetry requires H+/K+ ion flux, but is independent of Nodal signaling. Our pharmacological inhibitor studies show that ion flux is required for polarization of epidermal cilia and neurula rotation and suggest that ion flux functions synergistically with chorion contact to drive cardiac laterality. Live imaging analysis revealed that larval heart progenitor cells undergo a lateral shift without displaying any migratory behaviors. Furthermore, we find that this passive shift corresponds with the emergence of lateral asymmetry in the endoderm, which is also ion flux dependent. Our data suggest that ion flux promotes laterally asymmetric morphogenesis of the larval endoderm rudiment leading to a passive, Nodal-independent shift in the position of associated heart progenitor cells. These findings help to refine hypotheses regarding ancestral chordate left/right patterning mechanisms and how they have diverged within invertebrate and vertebrate chordate lineages. The online version of this article (doi:10.1186/s13227-017-0075-9) contains supplementary material, which is available to authorized users.
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DOI：
10.1073/pnas.1634991100
发表时间：
2003-09-30
影响因子：
11.1
作者：
Davidson, B;Levine, M
通讯作者：
Levine, M
影响因子：
9.2
作者：
Fischer, A;Viebahn, C;Blum, M
通讯作者：
Blum, M
影响因子：
3.7
作者：
Antic D;Stubbs JL;Suyama K;Kintner C;Scott MP;Axelrod JD
通讯作者：
Axelrod JD
影响因子：
0.7
作者：
Grande, Cristina;Maria Martin-Duran, Jose;Hejnol, Andreas
通讯作者：
Hejnol, Andreas
影响因子：
21.3
作者：
Borovina, Antonia;Superina, Simone;Ciruna, Brian
通讯作者：
Ciruna, Brian