The Retinoic Acid Receptors α and β are Expressed in the Human Promyelocytic Leukemia Cell Line HL-60
The Retinoic Acid Receptors α and β are Expressed in the Human Promyelocytic Leukemia Cell Line HL-60
复制标题
视黄酸受体 α 和 β 在人早幼粒细胞白血病细胞系 HL-60 中表达
DOI：
--
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
P. Chambon
中科院分区：
文献类型：
--
作者：
Y. Hashimoto;M. Petkovich;M. Gaub;H. Kagechika;K. Shudo;P. Chambon
人早幼粒白血病细胞系HL-60在维甲酸作用下可被诱导分化为粒细胞。此前，我们已经证明，未分化的HL-60细胞提取物具有特定的维甲酸结合活性(RSBP-1)，相当于大小排除层析测定的大约95千道尔顿(KDa)的蛋白质。我们现在将这些观察扩展到揭示第二个大约95 kDa的维甲酸结合成分(RSBP-2)，它可以通过阴离子交换层析从RSBP-1中分离出来。我们进一步证明，RSBP-1和RSBP-2的层析性质与分别转染人视黄酸受体(RAR)表达载体RAR-β∅和RAR-α∅的HeLa细胞提取物中的维甲酸结合活性相同。此外，一种针对RAR-β的抗血清制剂选择性地免疫沉淀了转染RAR-β∅的HeLa细胞提取物中的维甲酸结合活性以及与之相对应的维甲酸结合活性.
The human promyelocytic leukemia cell line HL-60 can be induced to differentiate into granulocytes upon exposure to retinoids. Previously we have shown that extracts of undifferentiated HL-60 cells possess a specific retinoid-binding activity (RSBP-1) corresponding to an approximate 95 kilodalton (kDa) protein as determined by size-exclusion chromatography. We now extend these observations to reveal a second approximate 95 kDa retinoic acidbinding component (RSBP-2), which is separable from RSBP-1 using anion exchange chromatography. We further show that the chromatographic properties of RSBP-1 and RSBP-2 are identical to those found for the retinoid-binding activities present in extracts of HeLa cells transfected with the human retinoic acid receptor (RAR) expression vectors RAR-β∅ and RAR-α∅, respectively. Moreover, an antiserum preparation directed against RAR-β selectively immunoprecipitated both the retinoid-binding activity in extracts of HeLa cells transfected with RAR-β∅) and that corresponding to...